Green silver nanoparticles of Phyllanthus amarus: as an antibacterial agent against multi drug resistant clinical isolates of Pseudomonas aeruginosa

Journal of Nanobiotechnology

Jaya Parkash Yadav²  Uma Chaudhary¹  Sangeeta Kadyan²  Manju Panghal²  Khushboo Singh² 

[1] Department of Microbiology, Pt. B.D.S Post Graduate Institute of Medical Sciences Rohtak, Rohtak 124001, Haryana, India;Department of Genetics, M.D. University, Rohtak 124001, Haryana, India

关键词: Antibacterial;    Silver nanoparticles;    Phyllanthus amarus;    MDR;    Burn isolates;    Pseudomonas aeruginosa;   

Others  :  1139433
DOI  :  10.1186/s12951-014-0040-x

 received in 2014-05-29, accepted in 2014-09-18,  发布年份 2014

【 摘 要 】

Background

Pseudomonas aeruginosa infection is a leading cause of morbidity and mortality in burn and immune-compromised patients. In recent studies, researchers have drawn their attention towards ecofriendly synthesis of nanoparticles and their activity against multidrug resistant microbes. In this study, silver nanoparticles were synthesized from aqueous extract of Phyllanthus amarus. The synthesized nanoparticles were explored as a potent source of nanomedicine against MDR burn isolates of P. aeruginosa.

Results

Silver nanoparticles were successfully synthesized using P. amarus extract and the nature of synthesized nanoparticles was analyzed by UV-Vis spectroscopy, transmission electron microscopy, energy dispersive X-ray spectroscopy, dynamic light scattering, zeta potential, X- ray diffraction and fourier transform infra-red spectroscopy. The average size of synthesized nanoparticles was 15.7, 24?±?8 and 29.78 nm by XRD, TEM and DLS respectively. The antibacterial activity of AgNPs was investigated against fifteen MDR strains of P. aeruginosa tested at different concentration. The zone of inhibition was measured in the range of 10?±?0.53 to 21?±?0.11mm with silver nanoparticles concentration of 12.5 to 100 ?g/ml. The zone of inhibition increased with increase in the concentration of silver nanoparticles. The MIC values of synthesized silver nanoparticles were found in the range of 6.25 to12.5 ?g/ml. The MIC values are comparable to the standard antibiotics.

Conclusion

The present study suggests that silver nanoparticles from P. amarus extract exhibited excellent antibacterial potential against multidrug resistant strains of P. aeruginosa from burn patients and gives insight of their potential applicability as an alternative antibacterial in the health care system to reduce the burden of multidrug resistance.

【 授权许可】

   
2014 Singh et al.; licensee BioMed Central Ltd.

【 图 表 】

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