Green silver nanoparticles of Phyllanthus amarus: as an antibacterial agent against multi drug resistant clinical isolates of Pseudomonas aeruginosa

Journal of Nanobiotechnology

Research

Khushboo Singh¹  Manju Panghal¹  Sangeeta Kadyan¹  Jaya Parkash Yadav¹  Uma Chaudhary² 

[1] Department of Genetics, M.D. University, 124001, Rohtak, Haryana, India;Department of Microbiology, Pt. B.D.S Post Graduate Institute of Medical Sciences Rohtak, 124001, Rohtak, Haryana, India;

关键词: Pseudomonas aeruginosa;    Burn isolates;    MDR;    Phyllanthus amarus;    Silver nanoparticles;    Antibacterial;   

DOI  :  10.1186/s12951-014-0040-x

 received in 2014-05-29, accepted in 2014-09-18,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundPseudomonas aeruginosa infection is a leading cause of morbidity and mortality in burn and immune-compromised patients. In recent studies, researchers have drawn their attention towards ecofriendly synthesis of nanoparticles and their activity against multidrug resistant microbes. In this study, silver nanoparticles were synthesized from aqueous extract of Phyllanthus amarus. The synthesized nanoparticles were explored as a potent source of nanomedicine against MDR burn isolates of P. aeruginosa.ResultsSilver nanoparticles were successfully synthesized using P. amarus extract and the nature of synthesized nanoparticles was analyzed by UV-Vis spectroscopy, transmission electron microscopy, energy dispersive X-ray spectroscopy, dynamic light scattering, zeta potential, X- ray diffraction and fourier transform infra-red spectroscopy. The average size of synthesized nanoparticles was 15.7, 24±8 and 29.78 nm by XRD, TEM and DLS respectively. The antibacterial activity of AgNPs was investigated against fifteen MDR strains of P. aeruginosa tested at different concentration. The zone of inhibition was measured in the range of 10±0.53 to 21±0.11mm with silver nanoparticles concentration of 12.5 to 100 µg/ml. The zone of inhibition increased with increase in the concentration of silver nanoparticles. The MIC values of synthesized silver nanoparticles were found in the range of 6.25 to12.5 µg/ml. The MIC values are comparable to the standard antibiotics.ConclusionThe present study suggests that silver nanoparticles from P. amarus extract exhibited excellent antibacterial potential against multidrug resistant strains of P. aeruginosa from burn patients and gives insight of their potential applicability as an alternative antibacterial in the health care system to reduce the burden of multidrug resistance.

【 授权许可】

Unknown   
© Singh et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.