Evaluation of basophil allergen threshold sensitivity (CD-sens) to peanut and Ara h 8 in children IgE-sensitized to Ara h 8

Clinical and Molecular Allergy

Anna Nopp¹  Magnus P Borres³  Magnus Wickman⁴  Anna Asarnoj²  S G O Johansson¹  Caroline Nilsson⁵  Susanne Glaumann⁵ 

[1] Department of Medicine, Clinical Immunology and Allergy Unit, Karolinska Institutet and Hospital, Stockholm, Sweden;Department of Women’s and Children’s Health, Karolinska Institutet at Astrid Lindgren Children’s Hospital, Stockholm, Sweden;Department of Women’s and Children’s Health, Uppsala University, Uppsala, Sweden;Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden;Sachs’ Children and Youth Hospital, Södersjukhuset, Stockholm, 118 83, Sweden

关键词: Pediatrics;    Peanut allergy;    Flow cytometry;    Cross-reactivity;    Children;    CD-sens;    CD63;    Birch pollen allergy;    Basophils;    rAra h 8;   

Others  :  1171968
DOI  :  10.1186/s12948-014-0007-3

 received in 2014-10-13, accepted in 2014-12-15,  发布年份 2015

【 摘 要 】

Background

Diagnosing peanut allergy properly is important and can be achieved by combining clinical history with various diagnostic methods such as IgE-antibody (IgE-ab) measurements, skin-prick test, basophil allergen threshold sensitivity (CD-sens) and food challenge. We aimed to evaluate CD-sens to peanut, Ara h 8 and Gly m 4 in relation to an oral peanut challenge in children IgE-sensitized to birch, peanut and Ara h 8 avoiding peanuts.

Methods

Twenty children IgE-sensitized to birch pollen and Ara h 8, but not to Ara h 1, Ara h 2 or Ara h 3 were challenged orally with roasted peanuts. Blood samples were drawn for IgE-ab and CD-sens analysis. To measure CD-sens, basophils were stimulated in vitro with decreasing doses of allergens until threshold sensitivity was reached.

Results

All children passed challenge without objective symptoms, but mild oral allergy syndrome (OAS) symptoms were reported in 6/20 children. Nineteen of twenty children were negative in CD-sens to peanut but 17/20 were positive to rAra h 8. Eleven of twenty children were positive in CD-sens to rGly m 4.

Conclusion

Positive CD-sens to rAra h 8 show that the Ara h 8 IgE-ab sensitized basophils can be activated by a rAra h 8 allergen and initiate an allergic inflammation despite a negative challenge. Hence, children sensitized to Ara h 8 but not to peanut storage proteins may be at risk for systemic allergic reaction when eating larger amounts of peanuts but most likely don’t have to fear smaller amounts.

【 授权许可】

   
2015 Glaumann et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

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