The 11S globulin Sin a 2 from yellow mustard seeds shows IgE cross-reactivity with homologous counterparts from tree nuts and peanut

Clinical and Translational Allergy

Oscar Palomares²  Mayte Villalba²  Rosalía Rodríguez²  Andrea Vereda¹  Javier Cuesta-Herranz¹  Martial Akotenou²  Sofía Sirvent² 

[1] Service of Allergy, IIS-Fundación Jiménez Díaz, Madrid, Spain;Department of Biochemistry and Molecular Biology, School of Chemistry, Complutense University of Madrid, Madrid, 28040, Spain

关键词: lgG/IgE epitopes;    Sin a 2;    11S globulins;    Cross-reactivity;    Peanut allergy;    Tree nut allergy;    Mustard allergy;    Food allergy;   

Others  :  794459
DOI  :  10.1186/2045-7022-2-23

 received in 2012-10-01, accepted in 2012-12-07,  发布年份 2012

【 摘 要 】

Background

The 11S globulin Sin a 2 is a marker to predict severity of symptoms in mustard allergic patients. The potential implication of Sin a 2 in cross-reactivity with tree nuts and peanut has not been investigated so far. In this work, we studied at the IgG and IgE level the involvement of the 11S globulin Sin a 2 in cross-reactivity among mustard, tree nuts and peanut.

Methods

Eleven well-characterized mustard-allergic patients sensitized to Sin a 2 were included in the study. A specific anti-Sin a 2 serum was obtained in rabbit. Skin prick tests (SPT), enzyme-linked immunosorbent assay (ELISA), immunoblotting and IgG or IgE-inhibition immunoblotting experiments using purified Sin a 2, Sin a 1, Sin a 3, mustard, almond, hazelnut, pistachio, walnut or peanut extracts were performed.

Results

The rabbit anti-Sin a 2 serum showed high affinity and specificity to Sin a 2, which allowed us to demonstrate that Sin a 2 shares IgG epitopes with allergenic 11S globulins from tree nuts (almond, hazelnut, pistachio and walnut) but not from peanut. All the patients included in the study had positive skin prick test to tree nuts and/or peanut and we subdivided them into two different groups according to their clinical symptoms after ingestion of such allergenic sources. We showed that 11S globulins contain conserved IgE epitopes involved in cross-reactivity among mustard, tree nuts and peanut as well as species-specific IgE epitopes.

Conclusions

The allergenic 11S globulin Sin a 2 from mustard is involved in cross-reactivity at the IgE level with tree nuts and peanut. Although the clinical relevance of the cross-reactive IgE epitopes present in 11S globulins needs to be investigated in further detail, our results contribute to improve the diagnosis and management of mustard allergic patients sensitized to Sin a 2.

【 授权许可】

   
2012 Sirvent et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Burks AW, Tang M, Sicherer S, Muraro A, Eigenmann PA, Ebisawa M, Fiocchi A, Chiang W, Beyer K, Wood R, et al.: ICON: food allergy. J Allergy Clin Immunol 2012, 129:906-920.
• [2]Chafen JJ, Newberry SJ, Riedl MA, Bravata DM, Maglione M, Suttorp MJ, Sundaram V, Paige NM, Towfigh A, Hulley BJ, Shekelle PG: Diagnosing and managing common food allergies: a systematic review. JAMA 2010, 303:1848-1856.
• [3]Akiyama H, Imai T, Ebisawa M: Japan food allergen labeling regulation-history and evaluation. Adv Food Nutr Res 2011, 62:139-171.
• [4]Monreal P, Botey J, Pena M, Marin A, Eseverri JL: Mustard allergy. Two anaphylactic reactions to ingestion of mustard sauce. Ann Allergy 1992, 69:317-320.
• [5]Panconesi E, Sertoli A, Fabbri P, Giorgini S, Spallanzani P: Anaphylactic shock from mustard after ingestion of pizza. Contact Dermatitis 1980, 6:294-295.
• [6]Stricker WE, Anorve-Lopez E, Reed CE: Food skin testing in patients with idiopathic anaphylaxis. J Allergy Clin Immunol 1986, 77:516-519.
• [7]Widstrom L, Johansson SG: IgE-mediated anaphylaxis to mustard. Acta Derm Venereol 1986, 66:70-71.
• [8]Kanny G, Fremont S, Talhouarne G, Nicolas JP, Moneret-Vautrin DA: Anaphylaxis to mustard as a masked allergen in “chicken dips”. Ann Allergy Asthma Immunol 1995, 75:340-342.
• [9]Directive 2006/142/EC of the European Parlament and of the Council of 29 Novemer 2006, amending directive 2000/13/EC. available at http://ec.europa.eu/food/labellingnutrition/foodlabelling/proposed_legislation_en.htm webcite
• [10]Menendez-Arias L, Moneo I, Dominguez J, Rodriguez R: Primary structure of the major allergen of yellow mustard (Sinapis alba L.) seed, Sin a I. Eur J Biochem 1988, 177:159-166.
• [11]Palomares O, Cuesta-Herranz J, Rodriguez R, Villalba M: A recombinant precursor of the mustard allergen Sin a 1 retains the biochemical and immunological features of the heterodimeric native protein. Int Arch Allergy Immunol 2005, 137:18-26.
• [12]Palomares O, Cuesta-Herranz J, Vereda A, Sirvent S, Villalba M, Rodriguez R: Isolation and identification of an 11S globulin as a new major allergen in mustard seeds. Ann Allergy Asthma Immunol 2005, 94:586-592.
• [13]Palomares O, Vereda A, Cuesta-Herranz J, Villalba M, Rodriguez R: Cloning, sequencing, and recombinant production of Sin a 2, an allergenic 11S globulin from yellow mustard seeds. J Allergy Clin Immunol 2007, 119:1189-1196.
• [14]Sirvent S, Palomares O, Vereda A, Villalba M, Cuesta-Herranz J, Rodriguez R: nsLTP and profilin are allergens in mustard seeds: cloning, sequencing and recombinant production of Sin a 3 and Sin a 4. Clin Exp Allergy 2009, 39:1929-1936.
• [15]Sirvent S, Palomares O, Cuesta-Herranz J, Villalba M, Rodriguez R: Analysis of the Structural and Immunological Stability of 2S Albumin, Nonspecific Lipid Transfer Protein, and Profilin Allergens from Mustard Seeds. J Agric Food Chem 2012, 60:6011-6018.
• [16]Vereda A, Sirvent S, Villalba M, Rodriguez R, Cuesta-Herranz J, Palomares O: Improvement of mustard (Sinapis alba) allergy diagnosis and management by linking clinical features and component-resolved approaches. J Allergy Clin Immunol 2011, 127:1304-1307.
• [17]Caballero T, San-Martin MS, Padial MA, Contreras J, Cabanas R, Barranco P, Lopez-Serrano MC: Clinical characteristics of patients with mustard hypersensitivity. Ann Allergy Asthma Immunol 2002, 89:166-171.
• [18]Figueroa J, Blanco C, Dumpierrez AG, Almeida L, Ortega N, Castillo R, Navarro L, Perez E, Gallego MD, Carrillo T: Mustard allergy confirmed by double-blind placebo-controlled food challenges: clinical features and cross-reactivity with mugwort pollen and plant-derived foods. Allergy 2005, 60:48-55.
• [19]Willison LN, Tripathi P, Sharma G, Teuber SS, Sathe SK, Roux KH: Cloning, expression and patient IgE reactivity of recombinant Pru du 6, an 11S globulin from almond. Int Arch Allergy Immunol 2011, 156:267-281.
• [20]Beyer K, Grishina G, Bardina L, Grishin A, Sampson HA: Identification of an 11S globulin as a major hazelnut food allergen in hazelnut-induced systemic reactions. J Allergy Clin Immunol 2002, 110:517-523.
• [21]Verweij MM, Hagendorens MM, De Knop KJ, Bridts CH, De Clerck LS, Stevens WJ, Ebo DG: Young infants with atopic dermatitis can display sensitization to Cor a 9, an 11S legumin-like seed-storage protein from hazelnut (Corylus avellana). Pediatr Allergy Immunol 2011, 22:196-201.
• [22]Koppelman SJ, Knol EF, Vlooswijk RA, Wensing M, Knulst AC, Hefle SL, Gruppen H, Piersma S: Peanut allergen Ara h 3: isolation from peanuts and biochemical characterization. Allergy 2003, 58:1144-1151.
• [23]Wallowitz M, Peterson WR, Uratsu S, Comstock SS, Dandekar AM, Teuber SS: Jug r 4, a legumin group food allergen from walnut (Juglans regia Cv. Chandler). J Agric Food Chem 2006, 54:8369-8375.
• [24]Ahn K, Bardina L, Grishina G, Beyer K, Sampson HA: Identification of two pistachio allergens, Pis v 1 and Pis v 2, belonging to the 2S albumin and 11S globulin family. Clin Exp Allergy 2009, 39:926-934.
• [25]Helm RM, Cockrell G, Connaughton C, Sampson HA, Bannon GA, Beilinson V, Nielsen NC, Burks AW: A soybean G2 glycinin allergen. 2. Epitope mapping and three-dimensional modeling. Int Arch Allergy Immunol 2000, 123:213-219.
• [26]Pastorello EA, Farioli L, Pravettoni V, Ispano M, Conti A, Ansaloni R, Rotondo F, Incorvaia C, Bengtsson A, Rivolta F, et al.: Sensitization to the major allergen of Brazil nut is correlated with the clinical expression of allergy. J Allergy Clin Immunol 1998, 102:1021-1027.
• [27]Teuber SS, Sathe SK, Peterson WR, Roux KH: Characterization of the soluble allergenic proteins of cashew nut (Anacardium occidentale L.). J Agric Food Chem 2002, 50:6543-6549.
• [28]Wallowitz ML, Chen RJ, Tzen JT, Teuber SS: Ses i 6, the sesame 11S globulin, can activate basophils and shows cross-reactivity with walnut in vitro. Clin Exp Allergy 2007, 37:929-938.
• [29]Sharma GM, Irsigler A, Dhanarajan P, Ayuso R, Bardina L, Sampson HA, Roux KH, Sathe SK: Cloning and characterization of an 11S legumin, Car i 4, a major allergen in pecan. J Agric Food Chem 2011, 59:9542-9552.
• [30]Manso L, Pastor C, Perez-Gordo M, Cases B, Sastre J, Cuesta-Herranz J: Cross-reactivity between coconut and lentil related to a 7S globulin and an 11S globulin. Allergy 2010, 65:1487-1488.
• [31]Varga EM, Kollmann D, Zach M, Bohle B: Anaphylaxis to buckwheat in an atopic child: a risk factor for severe allergy to nuts and seeds? Int Arch Allergy Immunol 2011, 156:112-116.
• [32]Ballabio C, Magni C, Restani P, Mottini M, Fiocchi A, Tedeschi G, Duranti M: IgE-mediated cross-reactivity among leguminous seed proteins in peanut allergic children. Plant Foods Hum Nutr 2010, 65:396-402.
• [33]Robotham JM, Hoffman GG, Teuber SS, Beyer K, Sampson HA, Sathe SK, Roux KH: Linear IgE-epitope mapping and comparative structural homology modeling of hazelnut and English walnut 11S globulins. Mol Immunol 2009, 46:2975-2984.
• [34]Guo F, Kothary MH, Wang Y, Yu X, Howard AJ, Fu TJ, Zhang YZ: Purification and crystallization of Cor a 9, a major hazelnut allergen. Acta Crystallogr Sect F Struct Biol Cryst Commun 2009, 65:42-46.
• [35]Comité de reacciones adversas a alimentos: Sociedad Española de Alergología e Inmunología Clínica. Metodología diagnóstica en la alergia a alimentos. Alergol Inmunol Clin 1999, 14:50-62.
• [36]Malling HJ: Skin prick testing in biological standardization of allergenic products. Arb Paul Ehrlich Inst Bundesamt Sera Impfstoffe Frankf AM 1997, 91:157-163.
• [37]Dreborg S: Skin testing. The safety of skin tests and the information obtained from using different methods and concentrations of allergen. Allergy 1993, 48:473-475.
• [38]Palomares O, Monsalve RI, Rodriguez R, Villalba M: Recombinant pronapin precursor produced in Pichia pastoris displays structural and immunologic equivalent properties to its mature product isolated from rapeseed. Eur J Biochem 2002, 269:2538-2545.
• [39]Sievers F, Wilm A, Dineen D, Gibson TJ, Karplus K, Li W, Lopez R, McWilliam H, Remmert M, Soding J, et al.: Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol 2011, 7:539.
• [40]Petersen TN, Brunak S, von Heijne G, Nielsen H: SignalP 4.0: discriminating signal peptides from transmembrane regions. Nat Methods 2011, 8:785-786.
• [41]Bordoli L, Kiefer F, Arnold K, Benkert P, Battey J, Schwede T: Protein structure homology modeling using SWISS-MODEL workspace. Nat Protoc 2009, 4:1-13.
• [42]Adachi M, Kanamori J, Masuda T, Yagasaki K, Kitamura K, Mikami B, Utsumi S: Crystal structure of soybean 11S globulin: glycinin A3B4 homohexamer. Proc Natl Acad Sci USA 2003, 100:7395-7400.
• [43]Palomares O, Alcantara M, Quiralte J, Villalba M, Garzon F, Rodriguez R: Airway disease and thaumatin-like protein in an olive-oil mill worker. N Engl J Med 2008, 358:1306-1308.
• [44]Valenta R, Twaroch T, Swoboda I: Component-resolved diagnosis to optimize allergen-specific immunotherapy in the Mediterranean area. J Investig Allergol Clin Immunol 2007, 17(Suppl 1):36-40.
• [45]Palomares O, Villalba M, Quiralte J, Polo F, Rodriguez R: 1,3-beta-glucanases as candidates in latex-pollen-vegetable food cross-reactivity. Clin Exp Allergy 2005, 35:345-351.
• [46]Bogh KL, Nielsen H, Madsen CB, Mills EN, Rigby N, Eiwegger T, Szepfalusi Z, Roggen EL: IgE epitopes of intact and digested Ara h 1: a comparative study in humans and rats. Mol Immunol 2012, 51:337-346.
• [47]Calderon MA, Demoly P, van Gerth Wijk R, Bousquet J, Sheikh A, Frew A, Scadding G, Bachert C, Malling HJ, Valenta R, et al.: EAACI: A European Declaration on Immunotherapy. Designing the future of allergen specific immunotherapy. Clin Transl Allergy 2012, 2:20. BioMed Central Full Text
• [48]Papadopoulos NG, Agache I, Bavbek S, Bilo BM, Braido F, Cardona V, Custovic A, Demonchy J, Demoly P, Eigenmann P, et al.: Research needs in allergy: an EAACI position paper, in collaboration with EFA. Clin Transl Allergy 2012, 2:21. BioMed Central Full Text