Heterogeneous responses and cross reactivity between the major peanut allergens Ara h 1, 2,3 and 6 in a mouse model for peanut allergy

Clinical and Translational Allergy

Raymond H Pieters¹  Els van Hoffen²  Manon van Roest¹  Karina Willemsen¹  Maarten T Pennings⁴  Joost J Smit³ 

[1] Immunotoxicology group, Institute for Risk Assessment Sciences, Utrecht University, Utrecht, The Netherlands;Current affiliation: NIZO food research BV, Ede, The Netherlands;Utrecht Centre for Food Allergy, Utrecht, The Netherlands;Current affiliation: HU University of Applied Sciences, Utrecht, The Netherlands

关键词: Anaphylaxis;    Sensitization;    Mouse model;    T cell;    Peanut proteins;    Peanut allergy;    IgE;    Cross-reactivity;   

Others  :  1149171
DOI  :  10.1186/s13601-015-0056-9

 received in 2015-01-16, accepted in 2015-02-28,  发布年份 2015

【 摘 要 】

Background

The relative contribution and the relation between individual peanut allergens in peanut allergic responses is still matter of debate. We determined the individual contribution of peanut proteins to B, T cell and allergic effector responses in a mouse model for peanut allergy.

Methods

Mice were immunized and challenged by oral gavage with peanut protein extract or isolated allergens Ara h 1, 2, 3 and 6 followed by assessment of food allergic manifestations. In addition, T cell responses to the individual proteins were measured by an in vitro dendritic cell-T cell assay.

Results

Sensitization with the individual peanut proteins elicited IgE responses with specificity to the allergen used as expected. However, cross reactivity among Ara h 1, 2, 3 and 6 was observed. T cell re-stimulations with peanut extract and individual peanut proteins also showed cross reactivity between Ara h 1, 2, 3 and 6. Despite the cross reactivity at the IgE level, only Ara h 2 and 6 were able to elicit mast cell degranulation after an oral challenge. However, after systemic challenge, Ara h 1, 2 and 6 and to lesser extent Ara h 3 were able to elicit anaphylactic responses.

Conclusions

Ara h 1, 2, 3 and 6 sensitize via the intra-gastric route, but differ in their capacity to cause allergic effector responses. Interestingly, extensive cross reactivity at T cell and antibody level is observed among Ara h 1, 2, 3 and 6, which may have important implications for the diagnosis and therapy of peanut allergy. Awareness about the relative contribution of individual peanut allergens and cross reactivity between these allergens is of importance for current research in diagnostics and therapeutics for and the mechanism of peanut allergy.

【 授权许可】

   
2015 Smit et al.; licensee BioMed Central.

【 图 表 】

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