Involvement of a periodontal pathogen, Porphyromonas gingivalis on the pathogenesis of non-alcoholic fatty liver disease

BMC Gastroenterology

Atsushi Nakajima⁷  Takashi Ooshima⁶  Takeshi Okanoue⁴  Atsuo Amano²  Yoshinori Kamisaki⁹  Kazuo Umemura³  Masae Kuboniwa²  Toshihide Shima⁴  Yoshio Sumida⁸  Iwai Tohnai¹  Shogo Murata¹  Masafumi Ono⁵  Kazuya Hokamura³  Ryota Nomura⁶  Kento Imajo⁷  Hironori Mawatari⁷  Hiroki Endo⁷  Koichiro Wada⁹  Kazuhiko Nakano⁶  Shuhei Naka⁶  Masato Yoneda⁷ 

[1] Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan;Department of Preventive Dentistry, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan;Department of Pharmacology, Hamamatsu University School of Medicine, Hamamatsu, Japan;Hepatology Center, Saiseikai Suita Hospital, Suita, Japan;Department of Gastroenterology and Hepatology, Kochi Medical School, Kochi, Japan;Department of Pediatric Dentistry, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan;Department of Gastroenterology, Yokohama City University Graduate School of Medicine, Yokohama, Japan;Center for Digestive and Liver Diseases, Nara City Hospital, Nara, Japan;Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan

关键词: Insulin resistance;    Oral bacteria;    P. gingivalis;    Non-alcoholic steatohepatitis (NASH);    Non-alcoholic fatty liver disease (NAFLD);   

Others  :  1113140
DOI  :  10.1186/1471-230X-12-16

 received in 2011-08-05, accepted in 2012-02-16,  发布年份 2012

【 摘 要 】

Background

Non-alcoholic fatty liver disease (NAFLD) is a hepatic manifestation of metabolic syndrome that is closely associated with multiple factors such as obesity, hyperlipidemia and type 2 diabetes mellitus. However, other risk factors for the development of NAFLD are unclear. With the association between periodontal disease and the development of systemic diseases receiving increasing attention recently, we conducted this study to investigate the relationship between NAFLD and infection with Porphyromonas gingivalis (P. gingivalis), a major causative agent of periodontitis.

Methods

The detection frequencies of periodontal bacteria in oral samples collected from 150 biopsy-proven NAFLD patients (102 with non-alcoholic steatohepatitis (NASH) and 48 with non-alcoholic fatty liver (NAFL) patients) and 60 non-NAFLD control subjects were determined. Detection of P. gingivalis and other periodontopathic bacteria were detected by PCR assay. In addition, effect of P. gingivalis-infection on mouse NAFLD model was investigated. To clarify the exact contribution of P. gingivalis-induced periodontitis, non-surgical periodontal treatments were also undertaken for 3 months in 10 NAFLD patients with periodontitis.

Results

The detection frequency of P. gingivalis in NAFLD patients was significantly higher than that in the non-NAFLD control subjects (46.7% vs. 21.7%, odds ratio: 3.16). In addition, the detection frequency of P. gingivalis in NASH patients was markedly higher than that in the non-NAFLD subjects (52.0%, odds ratio: 3.91). Most of the P. gingivalis fimbria detected in the NAFLD patients was of invasive genotypes, especially type II (50.0%). Infection of type II P. gingivalis on NAFLD model of mice accelerated the NAFLD progression. The non-surgical periodontal treatments on NAFLD patients carried out for 3 months ameliorated the liver function parameters, such as the serum levels of AST and ALT.

Conclusions

Infection with high-virulence P. gingivalis might be an additional risk factor for the development/progression of NAFLD/NASH.

【 授权许可】

   
2012 Yoneda et al; BioMed Central Ltd.

【 图 表 】

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