Involvement of a periodontal pathogen, Porphyromonas gingivalis on the pathogenesis of non-alcoholic fatty liver disease

BMC Gastroenterology

Research Article

Yoshio Sumida¹  Masafumi Ono²  Masato Yoneda³  Atsushi Nakajima³  Hiroki Endo³  Kento Imajo³  Hironori Mawatari³  Shogo Murata⁴  Iwai Tohnai⁴  Takashi Ooshima⁵  Kazuhiko Nakano⁵  Ryota Nomura⁵  Shuhei Naka⁵  Yoshinori Kamisaki⁶  Koichiro Wada⁷  Kazuya Hokamura⁸  Kazuo Umemura⁸  Masae Kuboniwa⁹  Atsuo Amano⁹  Toshihide Shima^1,10  Takeshi Okanoue^1,10 

[1] Center for Digestive and Liver Diseases, Nara City Hospital, Nara, Japan;Department of Gastroenterology and Hepatology, Kochi Medical School, Kochi, Japan;Department of Gastroenterology, Yokohama City University Graduate School of Medicine, Yokohama, Japan;Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan;Department of Pediatric Dentistry, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan;Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan;Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan;Department of Pharmacology, Graduate School of Dentistry, Osaka University, 1-8 Yamada-oka, Suita, 565-0871, Osaka, Japan;Department of Pharmacology, Hamamatsu University School of Medicine, Hamamatsu, Japan;Department of Preventive Dentistry, Graduate School of Dentistry, Osaka University, Suita, Osaka, Japan;Hepatology Center, Saiseikai Suita Hospital, Suita, Japan;

关键词: Non-alcoholic fatty liver disease (NAFLD);    Non-alcoholic steatohepatitis (NASH);    P. gingivalis;    Oral bacteria;    Insulin resistance;   

DOI  :  10.1186/1471-230X-12-16

 received in 2011-08-05, accepted in 2012-02-16,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundNon-alcoholic fatty liver disease (NAFLD) is a hepatic manifestation of metabolic syndrome that is closely associated with multiple factors such as obesity, hyperlipidemia and type 2 diabetes mellitus. However, other risk factors for the development of NAFLD are unclear. With the association between periodontal disease and the development of systemic diseases receiving increasing attention recently, we conducted this study to investigate the relationship between NAFLD and infection with Porphyromonas gingivalis (P. gingivalis), a major causative agent of periodontitis.MethodsThe detection frequencies of periodontal bacteria in oral samples collected from 150 biopsy-proven NAFLD patients (102 with non-alcoholic steatohepatitis (NASH) and 48 with non-alcoholic fatty liver (NAFL) patients) and 60 non-NAFLD control subjects were determined. Detection of P. gingivalis and other periodontopathic bacteria were detected by PCR assay. In addition, effect of P. gingivalis-infection on mouse NAFLD model was investigated. To clarify the exact contribution of P. gingivalis-induced periodontitis, non-surgical periodontal treatments were also undertaken for 3 months in 10 NAFLD patients with periodontitis.ResultsThe detection frequency of P. gingivalis in NAFLD patients was significantly higher than that in the non-NAFLD control subjects (46.7% vs. 21.7%, odds ratio: 3.16). In addition, the detection frequency of P. gingivalis in NASH patients was markedly higher than that in the non-NAFLD subjects (52.0%, odds ratio: 3.91). Most of the P. gingivalis fimbria detected in the NAFLD patients was of invasive genotypes, especially type II (50.0%). Infection of type II P. gingivalis on NAFLD model of mice accelerated the NAFLD progression. The non-surgical periodontal treatments on NAFLD patients carried out for 3 months ameliorated the liver function parameters, such as the serum levels of AST and ALT.ConclusionsInfection with high-virulence P. gingivalis might be an additional risk factor for the development/progression of NAFLD/NASH.

【 授权许可】

Unknown   
© Yoneda et al; BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.