Treadmill exercise enhances therapeutic potency of transplanted bone mesenchymal stem cells in cerebral ischemic rats via anti-apoptotic effects

BMC Neuroscience

Nan Liu³  Rong-Hua Chen³  Hou-Wei Du³  Long-Zai Lin³  Lin Cheng³  Ming-Zhou Yuan²  Yi-Xian Zhang¹ 

[1] Department of Rehabilitation, Union Hospital, Fujian Medical University, Fuzhou 350001, People’s Republic of China;College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, People’s Republic of China;Department of Neurology, Union Hospital, Fujian Medical University, Fuzhou 350001, People’s Republic of China

关键词: bcl-2;    Survivin;    Apoptosis;    Exercise;    Stroke;    MSCs;   

Others  :  1230652
DOI  :  10.1186/s12868-015-0196-9

 received in 2015-01-25, accepted in 2015-08-25,  发布年份 2015

【 摘 要 】

Background

The transplantation of bone marrow stromal cells (MSCs) has proved to ameliorate ischemic brain injury in animals, but most transplanted MSCs undergo apoptosis in the ischemic penumbra, greatly compromising the therapeutic value of this treatment. Meanwhile, cell apoptosis can be inhibited by post-ischemia exercise which has been demonstrated to improve the expression of related anti-apoptotic proteins. The present study investigated whether treadmill exercise enhances the neuroprotective effects of transplanted MSCs in a rat experimental stroke model.

Result

Rats were subjected to 2-h middle cerebral artery occlusion (MCAO). Twenty-four hours after reperfusion, they were assigned randomly to receive no MSCs treatment and no exercise (control group), intravenous transplantation of MSCs and treadmill exercise (MSCs + Ex group), MSCs transplantation only (MSCs group) and treadmill exercise only (Ex group). Neurological assessment, TUNEL staining and western blot were performed. Compared with the MSCs group and Ex group, the MSCs + Ex group reported markedly improved neurological function, significantly decreased apoptotic cells, and increased expressions of survivin and bcl-2 (p < 0.05 or p < 0.01, respectively). Interestingly, the treadmill exercise significantly inhibited the apoptosis of transplanted MSCs. As a result, the number of engrafted MSCs in the MSCs + Ex group was significantly higher than that in the MSC group (p < 0.01).

Conclusions

Treadmill exercise enhances the therapeutic potency of MSCs by improving neurological function and possibly inhibiting the apoptosis of neuron cells and transplanted MSCs. These effects may involve an increased expression of survivin and bcl-2.

【 授权许可】

   
2015 Zhang et al.

【 图 表 】

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