Activation of bcl‐2 suppressible 40 and 44 kDa p38‐like kinases during apoptosis of early and late B lymphocytic cell lines

FEBS Letters

Weissinger, Eva M⁶  Kolch, Walter¹  Mushinski, J.Frederic⁵  Baccarini, Manuela³  Henderson, Darren W⁵  Maucher, Cora¹  Wolff, Linda⁵  Procyk, Kasia³  Kaspers, Bernd²  Mischak, Harald¹  Kremmer, Elisabeth⁴ 

[1] Institut für Klinische Molekularbiologie und Tumorgenetik, GSF, Marchioninistr. 25, D-81377 Munich, Germany;Institut für Tierphysiologie, Ludwig-Maximilians-Universität München, D-80539 Munich, Germany;Institut für Mikrobiologie und Genetik der Universität Wien, 1030 Vienna, Austria;Institut für Immunologie, GSF, Marchioninistr. 25, D-81377 Munich, Germany;Laboratory of Genetics, National Cancer Institute, National Institutes of Health, Building 37, Room 2B26, NIH, 37 Convent Dr., MSC 4255, Bethesda, MD 20892, USA;Institut für Klinische Hämatologie, GSF, Marchioninistr. 25, D-81377 Munich, Germany

关键词: Apoptosis;    bcl-2;    Interleukin 1β converting enzyme;    Kinase;    p38;    Lymphocyte;    PKA;    protein kinase A;    MAP kinase;    mitogen activated kinase;    IL-6;    interleukin-6;    ICE;    interleukin 1β converting enzyme;   

DOI  :  10.1016/S0014-5793(98)00387-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Activation of several different kinases characterizes the induction of apoptosis. Abelson virus transformed pre-B lymphocytes undergo apoptosis within 24 h of serum deprivation, PKA activation or γ-irradiation, and the activity of two kinases of ca. 40 and 44 kDa is specifically induced during this apoptotic process. Bcl-2 expression prevents both apoptosis and the induction of these kinases. Immunologic and substrate similarities indicate that these kinases are related to the p38 family of MAP kinases. More mature cells of the B lymphocytic lineage, plasmacytomas, also exhibit induction of these kinases when apoptosis is induced by withdrawal of serum or IL-6. Treatment of the pre-B cells with ICE protease inhibitors when apoptotic stimuli are delivered prevents induction of the kinase activity, and partially inhibits apoptosis. These findings indicate that the induction of these 40 and 44 kDa p38 related kinases is a common feature of apoptosis in mouse B lymphocytic cells and may represent a step downstream of ICE proteases in the signal cascade that leads to programmed cell death.

【 授权许可】

Unknown