Treadmill exercise enhances therapeutic potency of transplanted bone mesenchymal stem cells in cerebral ischemic rats via anti-apoptotic effects

BMC Neuroscience

Research Article

Ming-Zhou Yuan¹  Long-Zai Lin²  Hou-Wei Du²  Lin Cheng²  Rong-Hua Chen²  Yi-Xian Zhang³  Nan Liu⁴ 

[1] College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, 350122, Fuzhou, People’s Republic of China;Department of Neurology, Union Hospital, Fujian Medical University, 350001, Fuzhou, People’s Republic of China;Department of Rehabilitation, Union Hospital, Fujian Medical University, 350001, Fuzhou, People’s Republic of China;Department of Rehabilitation, Union Hospital, Fujian Medical University, 350001, Fuzhou, People’s Republic of China;Department of Neurology, Union Hospital, Fujian Medical University, 350001, Fuzhou, People’s Republic of China;

关键词: MSCs;    Stroke;    Exercise;    Apoptosis;    Survivin;    bcl-2;   

DOI  :  10.1186/s12868-015-0196-9

 received in 2015-01-25, accepted in 2015-08-25,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe transplantation of bone marrow stromal cells (MSCs) has proved to ameliorate ischemic brain injury in animals, but most transplanted MSCs undergo apoptosis in the ischemic penumbra, greatly compromising the therapeutic value of this treatment. Meanwhile, cell apoptosis can be inhibited by post-ischemia exercise which has been demonstrated to improve the expression of related anti-apoptotic proteins. The present study investigated whether treadmill exercise enhances the neuroprotective effects of transplanted MSCs in a rat experimental stroke model.ResultRats were subjected to 2-h middle cerebral artery occlusion (MCAO). Twenty-four hours after reperfusion, they were assigned randomly to receive no MSCs treatment and no exercise (control group), intravenous transplantation of MSCs and treadmill exercise (MSCs + Ex group), MSCs transplantation only (MSCs group) and treadmill exercise only (Ex group). Neurological assessment, TUNEL staining and western blot were performed. Compared with the MSCs group and Ex group, the MSCs + Ex group reported markedly improved neurological function, significantly decreased apoptotic cells, and increased expressions of survivin and bcl-2 (p < 0.05 or p < 0.01, respectively). Interestingly, the treadmill exercise significantly inhibited the apoptosis of transplanted MSCs. As a result, the number of engrafted MSCs in the MSCs + Ex group was significantly higher than that in the MSC group (p < 0.01).ConclusionsTreadmill exercise enhances the therapeutic potency of MSCs by improving neurological function and possibly inhibiting the apoptosis of neuron cells and transplanted MSCs. These effects may involve an increased expression of survivin and bcl-2.

【 授权许可】

CC BY   
© Zhang et al. 2015