Antioxidant potential of Sutherlandia frutescens and its protective effects against oxidative stress in various cell cultures

BMC Complementary and Alternative Medicine

Nuran Ercal²  William R Folk¹  Connor J Hines²  Weili Fan²  Shakila Tobwala² 

[1] Department of Biochemistry, University of Missouri, Columbia, MO 65211, USA;Department of Chemistry, Missouri University of Science and Technology, 400 West 11th Street, 142 Schrenk Hall, Rolla, MO 65409, USA

关键词: Reactive oxygen species;    Radical scavenging;    Glutathione;    Antioxidant;    Oxidative stress;    Sutherlandia frutescens;   

Others  :  1087155
DOI  :  10.1186/1472-6882-14-271

 received in 2014-04-14, accepted in 2014-07-22,  发布年份 2014

【 摘 要 】

Background

Sutherlandia frutescens (L.) R.Br. (SF) is a South African plant that is widely used to treat stress, infections, cancer, and chronic diseases, many of which involve oxidative stress. The aim of the study was to quantitatively assess the antioxidant potential of SF extracts in cell-free system as well as in cell lines.

Methods

Dried SF vegetative parts were extracted using six different solvents, and the extracts were assessed for total phenolic and flavonoid contents, total reducing power, iron chelating capacity, and free radical scavenging power, including, scavenging of hydroxyl radicals, superoxide anions, nitric oxide, and hydrogen peroxide. We further investigated the freeze-dried hot water extract of SF (SFE) to assess its effect against oxidative stress induced by tert-butyl hydroperoxide (t-BHP), an organic peroxide. Three different cell lines: Chinese hamster ovary (CHO), human hepatoma (HepaRG), and human pulmonary alveolar carcinoma (A549) cells, were employed to determine cell viability, intracellular reactive oxygen species (ROS) levels, and reduced to oxidized glutathione levels (GSH/GSSG).

Results

The results indicated that: (1) SF extracts have significant antioxidant potential that is dependent upon the nature of the extraction solvent and (2) SFE protects against tBHP-induced oxidative stress in cells by scavenging ROS and preserving intracellular GSH/GSSG.

Conclusion

Oxidative stress is implicated in a number of disorders, and due to the public’s concerns about synthetic antioxidants, various natural antioxidants are being explored for their therapeutic potential. Our findings support claims for S. frutescens being a promising adjunctive therapeutic for oxidative stress-related health problems.

【 授权许可】

   
2014 Tobwala et al.; licensee BioMed Central Ltd.

【 图 表 】

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