Antioxidant potential of Sutherlandia frutescens and its protective effects against oxidative stress in various cell cultures

BMC Complementary and Alternative Medicine

Research Article

William R Folk¹  Weili Fan²  Connor J Hines²  Nuran Ercal²  Shakila Tobwala² 

[1] Department of Biochemistry, University of Missouri, 65211, Columbia, MO, USA;Department of Chemistry, Missouri University of Science and Technology, 400 West 11th Street, 142 Schrenk Hall, 65409, Rolla, MO, USA;

关键词: Sutherlandia frutescens;    Oxidative stress;    Antioxidant;    Glutathione;    Radical scavenging;    Reactive oxygen species;   

DOI  :  10.1186/1472-6882-14-271

 received in 2014-04-14, accepted in 2014-07-22,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundSutherlandia frutescens (L.) R.Br. (SF) is a South African plant that is widely used to treat stress, infections, cancer, and chronic diseases, many of which involve oxidative stress. The aim of the study was to quantitatively assess the antioxidant potential of SF extracts in cell-free system as well as in cell lines.MethodsDried SF vegetative parts were extracted using six different solvents, and the extracts were assessed for total phenolic and flavonoid contents, total reducing power, iron chelating capacity, and free radical scavenging power, including, scavenging of hydroxyl radicals, superoxide anions, nitric oxide, and hydrogen peroxide. We further investigated the freeze-dried hot water extract of SF (SFE) to assess its effect against oxidative stress induced by tert-butyl hydroperoxide (t-BHP), an organic peroxide. Three different cell lines: Chinese hamster ovary (CHO), human hepatoma (HepaRG), and human pulmonary alveolar carcinoma (A549) cells, were employed to determine cell viability, intracellular reactive oxygen species (ROS) levels, and reduced to oxidized glutathione levels (GSH/GSSG).ResultsThe results indicated that: (1) SF extracts have significant antioxidant potential that is dependent upon the nature of the extraction solvent and (2) SFE protects against tBHP-induced oxidative stress in cells by scavenging ROS and preserving intracellular GSH/GSSG.ConclusionOxidative stress is implicated in a number of disorders, and due to the public’s concerns about synthetic antioxidants, various natural antioxidants are being explored for their therapeutic potential. Our findings support claims for S. frutescens being a promising adjunctive therapeutic for oxidative stress-related health problems.

【 授权许可】

Unknown   
© Tobwala et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.