Quantitative analysis of Curcuminoid collected from different location in Indonesia by TLC-Densitometry and its antioxidant capacity

12th Joint Conference on Chemistry

Wahyuni, D.S.C.^1 ; Artanti, A.N.^1 ; Rinanto, Y.^2

Pharmacy Department, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Jl. Ir. Sutami 36 A, Central Java, Surakarta

57126, Indonesia^1

Biology Education Program, Faculty of Teacher Training and Education, Universitas Sebelas Maret, Jl. Ir. Sutami 36 A, Central Java, Surakarta

57126, Indonesia^2

关键词: Anti-oxidant activities;    Antioxidant capacity;    Bisdemethoxycurcumin;    Demethoxycurcumin;    Diphenylpicrylhydrazyl;    Pharmaceutical products;    Potent antioxidants;    Radical scavenging;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/349/1/012015/pdf
DOI  :  10.1088/1757-899X/349/1/012015

来源: IOP

【 摘 要 】

Curcuminoid, consisting of curcumin, demethoxycurcumin and bis demethoxycurcumin, is the active compound in Curcuma longa L. rhizome. It yielded vary depend on the environment and varieties. Curcumin has been known to have a potent antioxidants activity. The present work was conducted to assess the curcuminoid content and antioxidant capacity in the crude extracts of C. longa L. collected from eight different locations in Indonesia. Samples were collected from eight locations including Java island, Sumatera island and Nusa Tenggara island. All samples were extracted using 96% ethanol and analyzed quantitatively using TLC-Densitometry. Antioxidant activity was assessed using diphenylpicrylhydrazyl (DPPH) radical scavenging assay and analyzed at 517 nm using a spectrophotometer. Curcuminoid content in C. longa varied among eight different locations (0.53±0.05 - 5.33±0.12 % w/w). The highest curcumin, demethoxycurcumin and bisdemethoxycurcumin yield were found in the samples from Magetan (5.33±0.12%, 1.54±0.05%, 0.46±0.02% w/w, respectively). In contrast, the lowest curcumin, demethoxycurcumin and bisdemethoxycurcumin yield were found in the sample from Demak (0.53±0.05%, 0.17±0.05%, 0.17±0.05% w/w, respectively). Antioxidant capacity showed similar for all places unless sample from Magetan which exhibited two times lower than other locations. It is apparently curcuminoid content and antioxidant activity varied among places. These results are useful information for curcuminoid standardization method in pharmaceutical products.