Evidence of West Nile virus infection in Nepal

BMC Infectious Diseases

Julie A Pavlin⁴  Stefan Fernandez⁵  In-Kyu Yoon⁵  Samuel L Yingst⁵  Arjun Pant¹  Amod Bahadur Thapa²  Sanjaya Kumar Shrestha³  Chonticha Klungthong⁵  Piyawan Chinnawirotpisan⁵  Wiriya Rutvisuttinunt⁵ 

[1] Sukra Raj Tropical Infectious Diseases Hospital, Kathmandu, Nepal;Bharatpur Hospital, Chitwan, Nepal;Walter Reed/AFRIMS Research Unit Nepal, Kathmandu, Nepal;Armed Forces Health Surveillance Center, Silver Spring, Maryland, USA;Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

关键词: Phylogeny;    NGS;    Deep sequencing;    Febrile illness;    Nepal;    West nile virus;   

Others  :  1118426
DOI  :  10.1186/s12879-014-0606-0

 received in 2014-03-25, accepted in 2014-10-31,  发布年份 2014

【 摘 要 】

Background

Acute febrile illness is common among those seeking medical care and is frequently treated empirically with the underlying illness remaining undiagnosed in resource-poor countries. A febrile illness study was conducted 2009-2010 to identify known and unknown pathogens circulating in Nepal.

Method

Study methods included diagnostic testing and preliminary ELISA screening of acute and convalescent samples for diseases both known and unknown to be circulating in Nepal, including West Nile virus (WNV). The molecular assays including Polymerase Chain Reaction (PCR), Sanger sequencing and ultra deep sequencing on MiSeq Illumina Platform were conducted to further confirm the presence of WNV.

Results

The study enrolled 2,046 patients presenting undifferentiated febrile illness with unknown etiology. Sera from 14 out of 2,046 patients were tested positive for west nile virus (WNV) by nested Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Only two out of 14 cases were confirmed for the presence of WNV by sequencing and identified as WNV lineage 1 phylogentically. The two patients were adult males with fever and no neurological symptoms from Kathmandu and Bharatpur, Nepal.

Conclusion

Two out of 2,046 serum samples contained fragments of WNV genome resembling WNV lineage 1, which is evidence of the continued spread of WNV which should be considered a possible illness cause in Nepal.

【 授权许可】

   
2014 Rutvisuttinunt et al.; licensee BioMed Central Ltd.

【 图 表 】

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