Evidence of West Nile virus infection in Nepal

BMC Infectious Diseases

Julie A Pavlin⁴  Stefan Fernandez⁵  In-Kyu Yoon⁵  Samuel L Yingst⁵  Arjun Pant¹  Amod Bahadur Thapa²  Sanjaya Kumar Shrestha³  Chonticha Klungthong⁵  Piyawan Chinnawirotpisan⁵  Wiriya Rutvisuttinunt⁵ 

[1] Sukra Raj Tropical Infectious Diseases Hospital, Kathmandu, Nepal;Bharatpur Hospital, Chitwan, Nepal;Walter Reed/AFRIMS Research Unit Nepal, Kathmandu, Nepal;Armed Forces Health Surveillance Center, Silver Spring, Maryland, USA;Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand

关键词: Phylogeny;    NGS;    Deep sequencing;    Febrile illness;    Nepal;    West nile virus;   

Others  :  1118426
DOI  :  10.1186/s12879-014-0606-0

 received in 2014-03-25, accepted in 2014-10-31,  发布年份 2014

【 摘 要 】

Background

Acute febrile illness is common among those seeking medical care and is frequently treated empirically with the underlying illness remaining undiagnosed in resource-poor countries. A febrile illness study was conducted 2009-2010 to identify known and unknown pathogens circulating in Nepal.

Method

Study methods included diagnostic testing and preliminary ELISA screening of acute and convalescent samples for diseases both known and unknown to be circulating in Nepal, including West Nile virus (WNV). The molecular assays including Polymerase Chain Reaction (PCR), Sanger sequencing and ultra deep sequencing on MiSeq Illumina Platform were conducted to further confirm the presence of WNV.

Results

The study enrolled 2,046 patients presenting undifferentiated febrile illness with unknown etiology. Sera from 14 out of 2,046 patients were tested positive for west nile virus (WNV) by nested Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Only two out of 14 cases were confirmed for the presence of WNV by sequencing and identified as WNV lineage 1 phylogentically. The two patients were adult males with fever and no neurological symptoms from Kathmandu and Bharatpur, Nepal.

Conclusion

Two out of 2,046 serum samples contained fragments of WNV genome resembling WNV lineage 1, which is evidence of the continued spread of WNV which should be considered a possible illness cause in Nepal.

【 授权许可】

   
2014 Rutvisuttinunt et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Zanotto PM, Gould EA, Gao GF, Harvey PH, Holmes EC: Population dynamics of flaviviruses revealed by molecular phylogenies. Proc Natl Acad Sci U S A 1996, 93(2):548-553.
• [2]May FJ, Davis CT, Tesh RB, Barrett AD: Phylogeography of West Nile virus: from the cradle of evolution in Africa to Eurasia, Australia, and the Americas. J Virol 2011, 85(6):2964-2974.
• [3]Chowdhury P, Khan SA, Dutta P, Topno R, Mahanta J: Characterization of West Nile virus (WNV) isolates from Assam, India: insights into the circulating WNV in northeastern India. Comp Immunol Microbiol Infect Dis 2013, 37(1):39-47.
• [4]Li XL, Fu SH, Liu WB, Wang HY, Lu Z, Tong SX, Li ZX, Nasci RS, Kosoy O, Cui Y, Liang GD: West nile virus infection in Xinjiang. China Vector Borne Zoonotic Dis 2013, 13(2):131-133.
• [5]Joshi G, Pradhan S, Darsie J, Richard F: Culicine, Sabethine and Toxorhynchitine mosquitoes of Nepal including new country records. Entomological Society of Washington 1965, 67:137-146.
• [6]Pant GR, Lunt RA, Rootes CL, Daniels PW: Serological evidence for Japanese encephalitis and West Nile viruses in domestic animals of Nepal. Comp Immunol Microbiol Infect Dis 2006, 29(2–3):166-175.
• [7]Lanciotti RS, Kerst AJ, Nasci RS, Godsey MS, Mitchell CJ, Savage HM, Komar N, Panella NA, Allen BC, Volpe KE, Davis BS, Roehrig JT: Rapid detection of west nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay. J Clin Microbiol 2000, 38(11):4066-4071.
• [8]Shi PY, Kauffman EB, Ren P, Felton A, Tai JH, Dupuis AP 2nd, Jones SA, Ngo KA, Nicholas DC, Maffei J, Ebel GD, Bernard KA, Kramer LD: High-throughput detection of West Nile virus RNA. J Clin Microbiol 2001, 39(4):1264-1271.
• [9]Rutvisuttinunt W, Chinnawirotpisan P, Simasathien S, Shrestha SK, Yoon IK, Klungthong C, Fernandez S: Simultaneous and complete genome sequencing of influenza A and B with high coverage by Illumina MiSeq Platform. J Virol Methods 2013, 193(2):394-404.
• [10][http:/ / applications.illumina.com/ content/ dam/ illuminamarketing/ documents/ products/ appnotes/ appnote_afrims_rna_viruses.pdf] webcite Culture-Free Detection and Identification of Unknown RNA Viruses. .
• [11]Busch MP, Kleinman SH, Tobler LH, Kamel HT, Norris PJ, Walsh I, Matud JL, Prince HE, Lanciotti RS, Wright DJ, Linnen JM, Caglioti S: Virus and antibody dynamics in acute west nile virus infection. J Infect Dis 2008, 198(7):984-993.
• [12]Burt FJ, Grobbelaar AA, Leman PA, Anthony FS, Gibson GV, Swanepoel R: Phylogenetic relationships of southern African West Nile virus isolates. Emerg Infect Dis 2002, 8(8):820-826.
• [13]Erdelyi K, Ursu K, Ferenczi E, Szeredi L, Ratz F, Skare J, Bakonyi T: Clinical and pathologic features of lineage 2 West Nile virus infections in birds of prey in Hungary. Vector Borne Zoonotic Dis 2007, 7(2):181-188.
• [14]Bakonyi T, Ivanics E, Erdelyi K, Ursu K, Ferenczi E, Weissenbock H, Nowotny N: Lineage 1 and 2 strains of encephalitic West Nile virus, central Europe. Emerg Infect Dis 2006, 12(4):618-623.
• [15]Bondre VP, Jadi RS, Mishra AC, Yergolkar PN, Arankalle VA: West Nile virus isolates from India: evidence for a distinct genetic lineage. J Gen Virol 2007, 88(Pt 3):875-884.
• [16]Lvov DK, Butenko AM, Gromashevsky VL, Kovtunov AI, Prilipov AG, Kinney R, Aristova VA, Dzharkenov AF, Samokhvalov EI, Savage HM, Shchelkanov MY, Galkina IV, Deryabin PG, Gubler DJ, Kulikova LN, Alkhovsky SK, Moskvina TM, Zlobina LV, Sadykova GK, Shatalov AG, Lvov DN, Usachev VE, Voronina AG: West Nile virus and other zoonotic viruses in Russia: examples of emerging-reemerging situations. Arch Virol Suppl 2004, 18:85-96.
• [17]Takasaki T, Kotaki A, Nishimura K, Sato Y, Tokuda A, Lim CK, Ito M, Tajima S, Nerome R, Kurane I: Dengue virus type 2 isolated from an imported dengue patient in Japan: first isolation of dengue virus from Nepal. J Travel Med 2008, 15(1):46-49.