Adnectin Ligand Affinity Optimization With Dual Combinatorial Positional Scan Libraries

adnectin;ligand discovery;mRNA display;next-generation sequencing;NGS;affinity maturation;EGFR

Turechek, Luke J.

University:Havard University

Department:Biotechnology

关键词: adnectin;    ligand discovery;    mRNA display;    next-generation sequencing;    NGS;    affinity maturation;    EGFR;   

Others  :  https://dash.harvard.edu/bitstream/handle/1/42004030/TURECHEK-DOCUMENT-2018.pdf?sequence=1&isAllowed=y

美国|英语

来源: Digital Access to Scholarship at Harvard

【 摘 要 】

Discovery of antibody and alternative-scaffold ligands such as Adnectins to a protein target of interest has long been important for assay development and drug discovery.Affinity maturation of candidates with poor binding kinetics can hinder development in a fast-paced environment, with traditional methods being very time consuming, and faster methods involving single point mutation scanning being unlikely to yield highly improved variants.This thesis describes a method for the rapid discovery of affinity-matured variants of an EGFR-binding Adnectin using a chip oligo-based dual mutant library design in combination with mRNA display and next-generation sequencing.