Pharmacological, antioxidant, genotoxic studies and modulation of rat splenocyte functions by Cyperus rotundus extracts

BMC Complementary and Alternative Medicine

Chekir-Ghedira Leila²  Ghedira Kamel²  Limem Ilef¹  Ghedira Zied¹  Châbane Fadwa¹  Mhalla Dhekra¹  Kilani-Jaziri Soumaya¹ 

[1] Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03. Faculté de Pharmacie de Monastir, Université de Monastir, Rue Avicenne, Monastir, 5000, Tunisie;Laboratoire de Biologie Cellulaire et Moléculaire, Faculté de Médecine dentaire de Monastir, Université de Monastir, Rue Avicenne, Monastir, 5000, Tunisie

关键词: Immunomodulatory effect;    Chromosome aberrations;    Lipid peroxidation effect;    Anti-inflammatory activity;    Analgesic activity;    Cyperus rotundus;   

Others  :  1231122
DOI  :  10.1186/1472-6882-13-28

 received in 2012-09-26, accepted in 2013-01-17,  发布年份 2013

【 摘 要 】

Background

Cyperus rotundus Linn. (Cyperaceae) is a Tunisian medicinal plant used in folkloric (traditional) medicine to treat stomach disorders and inflammatory diseases. The present study explored the analgesic, anti-inflammatory and genotoxic activities of extracts from the aerial parts of C. rotundus. The antioxidant capacity and the modulation of splenocyte functions by these extracts were also investigated in mice. The phytochemical analysis was carried out using standard methods.

Methods

Aqueous, ethyl acetate, methanol and TOF-enriched extracts (300, 150, and 50 μg/ml) were evaluated for their analgesic and anti-inflammatory activities. 4, 2, and 1 mg/ml of each extract were tested to investigate their effect on lipid peroxidation. The genotoxic study was monitored by measuring the structural chromosome aberrations of mice treated with 300 mg/kg of extract. The proliferation of lymphocytes in the absence and presence of mitogens was assessed at a concentration range 1–1000 μg/ml.

Results

The tested extracts were able to decrease the mouse ear oedema induced by xylene. Furthermore, it was shown that the same extracts reduced the number of abdominal contractions caused by acetic acid in mice, revealing the peripheral analgesic activity of these extracts. It is worth noting that mice treated with doses up to 300 mg/kg b.w. of Cyperus rotundus extracts did not exhibit any toxicity. The tested extracts significantly enhance lymphocyte proliferation at 1 mg/ml.

Conclusions

It appears that C. rotundus extracts contain potent components such as flavonoids that may potentially be useful for modulating the immune cell functions, provoking analgesic, anti-inflammatory and antioxidant effects.

【 授权许可】

   
2013 Soumaya et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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