Pharmacological, antioxidant, genotoxic studies and modulation of rat splenocyte functions by Cyperus rotundus extracts

BMC Complementary and Alternative Medicine

Chekir-Ghedira Leila²  Ghedira Kamel²  Limem Ilef¹  Ghedira Zied¹  Châbane Fadwa¹  Mhalla Dhekra¹  Kilani-Jaziri Soumaya¹ 

[1]Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03. Faculté de Pharmacie de Monastir, Université de Monastir, Rue Avicenne, Monastir, 5000, Tunisie

[2]Laboratoire de Biologie Cellulaire et Moléculaire, Faculté de Médecine dentaire de Monastir, Université de Monastir, Rue Avicenne, Monastir, 5000, Tunisie

关键词: Immunomodulatory effect;    Chromosome aberrations;    Lipid peroxidation effect;    Anti-inflammatory activity;    Analgesic activity;    Cyperus rotundus;   

Others  :  1231122
DOI  :  10.1186/1472-6882-13-28

 received in 2012-09-26, accepted in 2013-01-17,  发布年份 2013

【 摘 要 】

Background

Cyperus rotundus Linn. (Cyperaceae) is a Tunisian medicinal plant used in folkloric (traditional) medicine to treat stomach disorders and inflammatory diseases. The present study explored the analgesic, anti-inflammatory and genotoxic activities of extracts from the aerial parts of C. rotundus. The antioxidant capacity and the modulation of splenocyte functions by these extracts were also investigated in mice. The phytochemical analysis was carried out using standard methods.

Methods

Aqueous, ethyl acetate, methanol and TOF-enriched extracts (300, 150, and 50 μg/ml) were evaluated for their analgesic and anti-inflammatory activities. 4, 2, and 1 mg/ml of each extract were tested to investigate their effect on lipid peroxidation. The genotoxic study was monitored by measuring the structural chromosome aberrations of mice treated with 300 mg/kg of extract. The proliferation of lymphocytes in the absence and presence of mitogens was assessed at a concentration range 1–1000 μg/ml.

Results

The tested extracts were able to decrease the mouse ear oedema induced by xylene. Furthermore, it was shown that the same extracts reduced the number of abdominal contractions caused by acetic acid in mice, revealing the peripheral analgesic activity of these extracts. It is worth noting that mice treated with doses up to 300 mg/kg b.w. of Cyperus rotundus extracts did not exhibit any toxicity. The tested extracts significantly enhance lymphocyte proliferation at 1 mg/ml.

Conclusions

It appears that C. rotundus extracts contain potent components such as flavonoids that may potentially be useful for modulating the immune cell functions, provoking analgesic, anti-inflammatory and antioxidant effects.

【 授权许可】

   
2013 Soumaya et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Pandurangan A, Khosa RL, Hemalatha S: Evaluation of anti-inflammatory and analgesic activity of root extract of Solanum trilobatum Linn. Iran J Pharmaceut Res 2008, 7:217-221.
• [2]Payne DO: Nitric oxide in allergic air way inflammation. Curr Opin Allergy Clin Immunol 2003, 3(2):133-137.
• [3]Hackett CJ: Innate immune activation as a broad-spectrum biodefense strategy: prospects and research challenges. J Allergy Clin Immunol 2003, 112:686-694.
• [4]Cuénod A: Flore de la Tunisie: cryptogames vasculaires, gymnospermes et monocotylèdones. Tunis: Office de l’expérimentation de la vulgarisation agricoles de Tunisie; 1954.
• [5]Gupta MB, Palit TK, Singh N, Bhargava KP: Pharmacological studies to isolate the active constituents from Cyperus rotundus possessing anti-inflammatory, anti-pyretic and analgesic activities. Indian J Med Res 1971, 59:76-82.
• [6]Seo WG, Pae HO, Oh GS, Chai KH, Kwon TA, Yun YG, Kim NY, Chung HT: Inhibitory effect of methanol extract of Cyperus rotundus rhizomes on nitric oxide and superoxide productions by murine macrophage cell line, RAW 264.7 cells. J Ethnopharmacol 2001, 76:59-64.
• [7]Singh N, Kulshrestha VK, Gupta MB, Bhargava KP: A pharmacological study of Cyperus rotundus. Indian J Med Res 1970, 58:103-109.
• [8]Zhu M, Luk HH, Fung HS, Luk CT: Cytoprotective effects of Cyperus rotundus against ethanol induced gastric ulceration in rats. Phytother Res 1997, 11:392-394.
• [9]Li GX: Pharmacology, Toxicity and Clinic of Traditional Chinese Medicine. Tianjin: Tianjin Science and Technique Translation Publishing House; 1992:194-195.
• [10]Vedavathy S, Rao KN: Antipyretic activity of six indigenous medicinal plants of Tirumala hills. India J Ethnopharmacol 1990, 33:193-196.
• [11]Yuan VY, Bone DE, Carrington FF: Antioxidant activity of dulse (Palmaria palmata) extract evaluated in vitro. Food Chem 2005, 91:485-494.
• [12]Chattopadhyay SK, Kumar S: Identification and quantification of two biologically active polyisoprenylated benzophenones xanthochymol and isoxanthochymol in Garcinia species using liquid chromatography-tandem mass spectrometry. J Chromatogr B 2006, 844:67-83.
• [13]Zhishen J, Mengcheng T, Jianming W: The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999, 64:555-559.
• [14]Pearson D: The Chemical Analysis of Foods. London: Churchill Livingstone; 1976.
• [15]Nwabueze TU: Effect of process variables on trypsin inhibitor activity (TIA) phytic acid and tannin content of extruded African breadfruit-corn-soy mixtures: a response surface analysis. Lebensm Wiss Technol 2007, 40:21-29.
• [16]Shibata S, Kumagai A, Harada M, Yano S, Saito H, Takahashi K: United States Patent. 1983, 4374284:7-10.
• [17]Tubaro A, Dri P, Delbello G, Zilli C, Delia LR: The croton oil ear test revisited. Agents Actions 1985, 17:347-349.
• [18]Asuzu IU, Sosa S, Delila LR: The anti-inflammatory activity of Icacina trichantha tuber. Phytomedicine 1999, 6:267-272.
• [19]Singh R, Singh B, Singh S, Kumar N, Kumar S, Arora S: Investigation of ethyl acetate extract/fractions of Acacia nilotica Willd. Ex Del as potent antioxidant. Rec Nat Prod 2009, 3:3:131-138.
• [20]Limem-Ben Amor I, Harizi H, Ghedira K, Chekir-Ghedira L: Leaf extracts from Phlomis crinita Cav. subs. mauritanica Munby affect immune cell functions in vitro. Immunopharmacol Immunotoxicol 2011, 33:309-314.
• [21]Mossmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983, 65:55-63.
• [22]Zorgui L, Boussema YI, Ayed Y, Bacha H, Hassen W: The antigenotoxic activities of cactus (Opuntia ficus-indica) cladodes against the mycotoxin zearalenone in Balb/c mice: prevention of micronuclei, chromosome aberrations and DNA fragmentation. Food Chem Toxicol 2009, 47:662-667.
• [23]Evans EP, Breckon G, Ford CE: An air drying method for meiotic preparation from mammalian tests. Cytogenetics 1960, 3:613-616.
• [24]Abbès S, Ouanes Z, Ben Salah-Abbès J, Abdel-Wahhab MA, Oueslati R, Bacha H: Preventive role of aluminosilicate clay against induction of micronuclei and chromosome aberrations in bone-marrow cells of Balb/c mice treated with Zearalenone. Mutat Res 2007, 631:85-92.
• [25]Savage JRK: Classification and relationship of induced chromosomal structural changes. J Med Genet 1975, 12:103-122.
• [26]Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen M: Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem 1999, 47:3954-3962.
• [27]Capecka EM, ALeja M: Antioxidant activity of fresh and dry herbs of some Lamiaceae species. Food Chem 2005, 93:223-226.
• [28]Braughler JM, Duncan LA, Chase RL: The involvement of iron in lipid peroxidation. Imporatnce of ferric to ferrous iron in initiation. J Biol Chem 1986, 261:10282-10289.
• [29]Gutteridge JMC: Age pigments and free radicals: fluorescent lipid complexes formed by iron and copper containing proteins. Biochem Biophys Acta 1985, 834:144-151.
• [30]Draper HH, Hadley M: Malondialdehyde determination as an index of lipid peroxidation. Methods Enzymol 1990, 186:421-431.
• [31]Manosroi A, Saraphanchotiwitthaya A, Manosroi J: In vitro immunomodulatory effect of Pouteria cambodiana (Pierre ex Dubard) Baehni extract. J Ethnopharmacol 2005, 101:90-94.
• [32]Han SB, Kim YH, Lee CW, Park SM, Lee HY, Ahn KS, Kim IH, Kim HM: Characteristic immunostimulation by angelan isolated from Angelica. Int Immunopharmacol 1998, 40:39-48.
• [33]Kilani-Jaziri S, Neffati A, Limem I, Boubaker J, Skandrani I, Ben Sghair M, Bouhlel I, Bhouri W, Mariotte AM, Ghedira K, Dijoux Franca MG, Chekir-Ghedira L: Relationship correlation of antioxidant and antiproliferative capacity of Cyperus rotundus products towards K562 erythroleukemia cells. Chem Biol Interact 2009, 181:85-94.
• [34]Parmar NS, Ghosh MMN: Current trends in flavonoid research. Indian J Pharmacol 1978, 12:213-228.
• [35]Ramesh M, Rao YN, Rao AV, Prabhakar MC, Rao CS, Muralidhar N, Reddy BM: Antinociceptive and anti-inflammatory activity of a flavonoid isolated from Caralluma attenuata. J Ethnopharmacol 1998, 62:63-66.
• [36]Nunez-Guillèn ME, da Silva Emin JA, Souccar C, Lapa AJ: Analgesic and anti-inflammatory activities of the aqueous extract of Plantago major L. Int J Pharmacogn 1997, 35:99-104.
• [37]Adeyemia OO, Yemitan OK, Afolabi L: Inhibition of chemically induced inflammation and pain by orally and topically administered leaf extract of Manihot esculenta Crantz in rodents. J Ethnopharmacol 2008, 119:6-11.
• [38]Zhang L, Hu JJ, Lin JW, Fang WS, Du GH: Anti-inflammatory and analgesic effects of ethanol and aqueous extracts of Pterocephalus hookeri (C.B. Clarke) Hoeck. J Ethnopharmacol 2009, 123:510-514.
• [39]Tsung-Chun L, Yu-Zen K, Hsin-Wei H, Ying-Chun H, Ying-Chih L, Wen- Huang P: Analgesic and anti-inflammatory activities of aqueous extract from Glycine tomentella root in mice. J Ethnopharmacol 2007, 113:142-148.
• [40]Ferreira SH, Vane JR: New aspects in the mode of action of non-steroidal anti-inflammatory drugs. Annu Rev Pharmacol 1974, 14:57-73.
• [41]Berkenkopf JW, Weichman BM: Production of prostacyclin in mice following intraperitoneal injection of acetic acid, phenylbenzoquinone and zymosan: its role in the writhing response. Prostaglandins 1988, 36:693-709.
• [42]Santos ARS, Filho VC, Niero R, Viana AM, Morenof N, Campos MM, Yunes RA, Calixto JB: Analgesic effects of callus culture from selected species of Phyllantus in mice. J Pharm Pharmacol 1994, 46:755-759.
• [43]Andrade SF, Cardoso LGV, Carvalho JCT, Basteos JK: Anti-inflammatory and antinociceptive activities of extract, fractions and populnoic acid from bark wood of Austroplenckia populnea. J Ethnopharmacol 2007, 109:464-471.
• [44]Young JM, Young L: Pharmacological Methods in the Control of Inflammation. Edited by Spector J, Back N. New York: Alan R. Li; 1989:215-231.
• [45]Atta AH, Alkohafi A: Antinociceptive and anti-inflammatory effects of some Jondanian medicinal plants extracts. J Ethnopharmacol 1998, 60:117-124.
• [46]Okoli ACO, Akah PA, Nwafor SV, Anisiobi AI, Ibegbunam IN, Erojikwe O: Anti-inflammatory activity of hexane leaf extract of Aspilia africana C.D. J Ethnopharmacol 2007, 109:219-225.
• [47]Ahmed MM, Qureshi S, Al-Bekairi AM, Shah A, Rao RM, Qazi NS: Anti-inflammatory activity of Caralluma tuberculata alcoholic extract. Fitoterapia 1993, 4:357-360.
• [48]Calixto JB, Beirith A, Ferreira J, Santos AR, Cechinel Filho V, Yunes RA: Naturally occurring antinociceptive substances from plants. Phytother Res 2000, 14:401-418.
• [49]Kilani S, Ben Ammar R, Bouhlel I, Abdelwahed A, Hayder N, Ghedira-Chekir L: Investigation of extracts from (Tunisian) Cyperus rotundus as antimutagens and radical scavengers. Environ Toxicol Pharmacol 2005, 20:478-484.
• [50]Akihisa T, Yasukawa K, Kimura Y, Takase S, Yamanouchi S, Tamura T: Triterpene alcohols from camellia and sasanqua oils and their anti-inflammatory effects. Chem Pharm Bull 1997, 45:2016-2023.
• [51]Park EH, Kahng JH, Lee SH, Shin KH: Anti-inflammatory principles from Cactus. Fitoterapia 2001, 72:288-290.
• [52]Chen TL, Chang CC, Lin YL, Ueng YF, Chen RM: Signal-transducing mechanisms of ketamine-caused inhibition of interleukin-1 gene expression in lipopolysaccharide-stimulated murine macrophage-like Raw 264.7 cells. Toxicol Appl Pharmacol 2009, 240:15-25.
• [53]Oyanagui Y: Various actions of active oxygens on inflammatory process. Jpn J Inflamm 1983, 3:377-378.
• [54]Sangameswaran B, Balakrishnan BR, Chumbhale D, Jayakar B: In vitro antioxidant activity of roots of Thespesia lampas Dalz and Gibs. Pak J Pharm Sci 2009, 22:368-372.
• [55]Rios JL, Manèz S, Paya M, Alcaraz MJ: Antioxidant activity of flavonoids from Sideritis javalambrensis. Phytochemistry 1992, 31:1947-1950.
• [56]Swierenga SHH, Heddle JA, Sigal EA, Gilman JPW, Brillinger RL, Douglas GR, Nestmann ER: Recommended protocols based on a survey of current practice in genotoxicity testing laboratories. IV. Chromosome aberrations and sister-chromatid exchange in Chinese hamster ovary, V79 Chinese hamster lung and human lymphocyte cultures. Mutat Res 1991, 246:301-322.