Regulation of microRNAs miR-30a and miR-143 in cerebral vasculature after experimental subarachnoid hemorrhage in rats

BMC Genomics

Lars Edvinsson¹  Karin Warfvinge¹  Janne Nielsen¹  Lars Schack Kruse¹  Claus Heiner Bang-Berthelsen¹  Gro Klitgaard Povlsen¹  Anne Holt Müller¹ 

[1] Department of Clinical Experimental Research, Glostrup Research Institute, Glostrup University Hospital, Nordre Ringvej 69, Glostrup 2600, Denmark

关键词: Non-coding RNA;    Biomarker;    Artery;    Animal model;    SAH;   

Others  :  1131240
DOI  :  10.1186/s12864-015-1341-7

 received in 2014-09-08, accepted in 2015-02-12,  发布年份 2015

【 摘 要 】

Background

microRNAs (miRNAs) are important regulators of translation and have been implicated in the pathogenesis of a number of cardiovascular diseases, including stroke, and suggested as possible prognostic biomarkers. Our aim was to identify miRNAs that are differentially regulated in cerebral arteries after subarachnoid hemorrhage (SAH), using a rat injection model of SAH and a qPCR-based screen of 728 rat miRNAs. Additionally, serum was analyzed for a possible spill-over to the circulation of regulated miRNAs from the vessel walls.

Results

We identified 482 different miRNAs expressed in cerebral arteries post-SAH. Two miRNAs, miR-30a and miR-143, were significantly upregulated in cerebral arteries after SAH when compared to sham-operated animals. However, none of these exhibited significantly altered serum levels after SAH versus post-sham surgery. The most robust upregulation was seen for miR-143, which has several predicted targets and is a strong regulator of vascular morphology. We hypothesize that miR-30a and miR-143 may play a role in the vascular wall changes seen after SAH.

Conclusions

We report that miR-30a and miR-143 in the cerebral arteries show significant changes over time after SAH, but do not differ from sham-operated rats at 24 h post-SAH. Although this finding suggests interesting novel possible mechanisms involved in post-SAH cerebrovascular changes, the lack of regulation of these miRNAs in serum excludes their use as blood-borne biomarkers for cerebrovascular changes following SAH.

【 授权许可】

   
2015 Müller et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

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