Occurrence of the invasion associated marker (iam) in Campylobacter jejuni isolated from cattle

BMC Research Notes

Gireesh Rajashekara²  Jeffrey T LeJeune²  Jun Lin¹  Zhe Liu²  Issmat I Kassem²  Yasser M Sanad² 

[1] Department of Animal Science, The University of Tennessee, Knoxville, TN 37996, USA;Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH 44691, USA

关键词: Invasion associated marker (iam);    Host colonization;    Invasion;    Cattle;    Campylobacter jejuni;   

Others  :  1166825
DOI  :  10.1186/1756-0500-4-570

 received in 2011-11-19, accepted in 2011-12-30,  发布年份 2011

【 摘 要 】

Background

The invasion associated marker (iam) has been detected in the majority of invasive Campylobacter jejuni retrieved from humans. Furthermore, the detection of iam in C. jejuni isolated from two important hosts, humans and chickens, suggested a role for this marker in C. jejuni's colonization of multiple hosts. However, no data exist regarding the occurrence of this marker in C. jejuni isolated from non-poultry food-animals such as cattle, an increasingly important source for human infections. Since little is known about the genetics associated with C. jejuni's capability for colonizing physiologically disparate hosts, we investigated the occurrence of the iam in C. jejuni isolated from cattle and assessed the potential of iam-containing cattle and human isolates for chicken colonization and human cell invasion.

Results

Simultaneous RAPD typing and iam-specific PCR analysis of 129 C. jejuni isolated from 1171 cattle fecal samples showed that 8 (6.2%) of the isolates were iam-positive, while 7 (54%) of human-associated isolates were iam-positive. The iam sequences were mostly heterogeneous and occurred in diverse genetic backgrounds. All iam-positive isolates were motile and possessed important genes (cadF, ciaB, cdtB) associated with adhesion and virulence. Although certain iam-containing isolates invaded and survived in INT-407 cells in high numbers and successfully colonized live chickens, there was no clear association between the occurrence, allelic sequence, and expression levels of the iam and the aforementioned phenotypes.

Conclusions

We show that the prevalence of iam in cattle C. jejuni is relatively lower as compared to isolates occurring in humans and chickens. In addition, iam was polymorphic and certain alleles occur in cattle isolates that were capable of colonizing and invading chickens and human intestinal cells, respectively. However, the iam did not appear to contribute to the cattle-associated C. jejuni's potential for invasion and intracellular survival in human intestinal cells as well as chicken colonization.

【 授权许可】

   
2011 Sanad et al; licensee BioMed Central Ltd.

【 图 表 】

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