Protective effect of Danggui (Radix Angelicae Sinensis) on angiotensin II-induced apoptosis in H9c2 cardiomyoblast cells

BMC Complementary and Alternative Medicine

Dennis Jine-Yuan Hsieh¹  Peng-Yu Liu³  Fuu-Jen Tsai³  Chia-Hua Kuo⁵  Wei-Wen Kuo⁴  Chih-Yang Huang² 

[1] Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan;Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan;Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan;Department of Biological Science and Technology, China Medical University, Taichung, Taiwan;Department of Sports Sciences, University of Taipei, Taipei, Taiwan

关键词: Apoptosis;    Cardiomyoblast;    Angiotensin II;    Radix Angelicae Sinensis;    Danggui;    Traditional Chinese Medicine (TCM);   

Others  :  1086539
DOI  :  10.1186/1472-6882-14-358

 received in 2014-04-11, accepted in 2014-09-19,  发布年份 2014

【 摘 要 】

Background

Danggui (Radix Angelicae Sinensis) is an herb often used in Traditional Chinese medicine. It is used to promote blood flow and has been used in the treatment of myocardial ischemia-reperfusion injury in animal models. Angiotensin II (Ang II) has been shown to play important roles in mediating cardiovascular diseases, and may cause cardiac hypertrophy and apoptosis. This study aimed to investigate whether Danggui has protective effects on Ang II-induced apoptosis in H9c2 cardiomyoblast cells and study the mechanisms involved.

Methods

We evaluated the effect of Danggui on Ang II-induced apoptosis in an in vitro model. H9c2 cardiomyoblast cells were cultured in serum-free medium for 4 hr, then treated with Danggui (50, 100 μg/ml) 1 hr pre- or post-Ang II treatment. After a further 23 hr of culture, cells were harvested for analyses with assays for apoptosis markers and cell signaling pathways.

Results

Our results showed that Ang II induced upregulation of pro-apoptotic Bad, instability of the mitochondria membrane potential, cytochrome c release, caspase-9 and caspase-3 activation and cardiomyocyte apoptosis. Pre- or post-treatment with Danggui reversed all of the above Ang II-induced apoptotic effects in H9c2 cells. Furthermore, the JNK (SP600125) inhibitor completely blocked Danggui inhibition of caspase-3 activation in Ang II-treated H9c2 cells.

Conclusions

Our results showed that Danggui either pre-treatment or post-treatment highly attenuated the Ang II-induced apoptosis in cardiomyoblast cells. The findings demonstrated that the anti-apoptosis effect of Danggui is mediated by JNK and PI3k inhibitors.

【 授权许可】

   
2014 Huang et al.; licensee BioMed Central Ltd.

【 图 表 】

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