Protective effect of Danggui (Radix Angelicae Sinensis) on angiotensin II-induced apoptosis in H9c2 cardiomyoblast cells

BMC Complementary and Alternative Medicine

Research Article

Wei-Wen Kuo¹  Chia-Hua Kuo²  Fuu-Jen Tsai³  Peng-Yu Liu³  Chih-Yang Huang⁴  Dennis Jine-Yuan Hsieh⁵ 

[1]Department of Biological Science and Technology, China Medical University, Taichung, Taiwan

[2]Department of Sports Sciences, University of Taipei, Taipei, Taiwan

[3]Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan

[4]Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan

[5]Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan

[6]Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan

[7]School of Medical Laboratory and Biotechnology, Chung Shan Medical University, No.110, Sec.1, Jianguo N. Rd, 40201, Taichung, Taiwan

[8]Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan

关键词: Traditional Chinese Medicine (TCM);    Danggui;    Radix Angelicae Sinensis;    Angiotensin II;    Cardiomyoblast;    Apoptosis;   

DOI  :  10.1186/1472-6882-14-358

 received in 2014-04-11, accepted in 2014-09-19,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundDanggui (Radix Angelicae Sinensis) is an herb often used in Traditional Chinese medicine. It is used to promote blood flow and has been used in the treatment of myocardial ischemia-reperfusion injury in animal models. Angiotensin II (Ang II) has been shown to play important roles in mediating cardiovascular diseases, and may cause cardiac hypertrophy and apoptosis. This study aimed to investigate whether Danggui has protective effects on Ang II-induced apoptosis in H9c2 cardiomyoblast cells and study the mechanisms involved.MethodsWe evaluated the effect of Danggui on Ang II-induced apoptosis in an in vitro model. H9c2 cardiomyoblast cells were cultured in serum-free medium for 4 hr, then treated with Danggui (50, 100 μg/ml) 1 hr pre- or post-Ang II treatment. After a further 23 hr of culture, cells were harvested for analyses with assays for apoptosis markers and cell signaling pathways.ResultsOur results showed that Ang II induced upregulation of pro-apoptotic Bad, instability of the mitochondria membrane potential, cytochrome c release, caspase-9 and caspase-3 activation and cardiomyocyte apoptosis. Pre- or post-treatment with Danggui reversed all of the above Ang II-induced apoptotic effects in H9c2 cells. Furthermore, the JNK (SP600125) inhibitor completely blocked Danggui inhibition of caspase-3 activation in Ang II-treated H9c2 cells.ConclusionsOur results showed that Danggui either pre-treatment or post-treatment highly attenuated the Ang II-induced apoptosis in cardiomyoblast cells. The findings demonstrated that the anti-apoptosis effect of Danggui is mediated by JNK and PI3k inhibitors.

【 授权许可】

Unknown   
© Huang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.