Influence of heat shock and osmotic stresses on the growth and viability of Saccharomyces cerevisiae SUBSC01

BMC Research Notes

Rashed Noor¹  Sanjida Humayun¹  Md. Sakil Munna¹ 

[1] Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

关键词: Viable and culturable cells;    Budding yeasts;    Critical growth temperature;    Saccharomyces cerevisiae;    Osmotic stress;    Heat stress;   

Others  :  1230859
DOI  :  10.1186/s13104-015-1355-x

 received in 2014-11-20, accepted in 2015-08-17,  发布年份 2015

【 摘 要 】

Background

With a preceding scrutiny of bacterial cellular responses against heat shock and oxidative stresses, current research further investigated such impact on yeast cell. Present study attempted to observe the influence of high temperature (44–46 °C) on the growth and budding pattern of Saccharomyces cerevisiae SUBSC01. Effect of elevated sugar concentrations as another stress stimulant was also observed. Cell growth was measured through the estimation of the optical density at 600 nm (OD ₆₀₀ ) and by the enumeration of colony forming units on the agar plates up to 450 min.

Results

Subsequent transformation in the yeast morphology and the cellular arrangement were noticed. A delayed and lengthy lag phase was observed when yeast strain was grown at 30, 37, and 40 °C, while at 32.5 °C, optimal growth pattern was noticed. Cells were found to lose culturability completely at 46 °C whereby cells without the cytoplasmic contents were also observed under the light microscope. Thus the critical growth temperature was recorded as 45 °C which was the highest temperature at which S. cerevisiae SUBSC01 could grow. However, a complete growth retardation was observed at 45 °C with the high concentrations of dextrose (0.36 g/l) and sucrose (0.18 g/l). Notably, yeast budding was found at 44 and 45 °C up to 270 min of incubation, which was further noticed to be suppressed at 46 °C.

Conclusions

Present study revealed that the optimal and the critical growth temperatures of S. cerevisiae SUBSC01 were 32.5 and 45 °C, respectively; and also projected on the inhibitory concentrations of sugars on yeast growth at that temperature.

【 授权许可】

   
2015 Munna et al.

【 图 表 】

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