Tolerance of budding yeast Saccharomyces cerevisiae to ultra high pressure

18th APS-SCCM; 24th AIRAPT

Shibata, M.^1 ; Torigoe, M.^1 ; Matsumoto, Y.^1 ; Yamamoto, M.^1 ; Takizawa, N.^2 ; Hada, Y.^3 ; Mori, Y.^4 ; Takarabe, K.^4 ; Ono, F.^4

Okayama Ichinomiya Senior High School, 221 Narazu, Kitaku, Okayama

701-1202, Japan^1

Department of Applied Chemistry and Biotechnology, Okayama University of Science, 1-1 Ridaicho, Kitaku, Okayama

700-0005, Japan^2

Department of Biosphere-Geosphere System Science, Okayama University of Science, 1-1 Ridaicho, Kitaku, Okayama

700-0005, Japan^3

Department of Applied Science, Okayama University of Science, 1-1 Ridaicho, Kitaku, Okayama

700-0005, Japan^4

关键词: Budding yeasts;    Exposed to;    High pressure;    High-pressure tolerance;    Liquid pressure;    Potato dextrose agars;    Ultrahigh pressure;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/500/10/102004/pdf
DOI  :  10.1088/1742-6596/500/10/102004

来源: IOP

【 摘 要 】

Our studies on the tolerance of plants and animals against very high pressure of several GPa have been extended to a smaller sized fungus, the budding yeast Saccharomyces cerevisiae. Several pieces of budding yeast (dry yeast) were sealed in a small teflon capsule with a liquid pressure medium fluorinate, and exposed to 7.5 GPa by using a cubic anvil press. The pressure was kept constant for various duration of time from 2 to 24 h. After the pressure was released, the specimens were brought out from the teflon capsule, and they were cultivated on a potato dextrose agar. It was found that the budding yeast exposed to 7.5 GPa for up to 6 h showed multiplication. However, those exposed to 7.5 GPa for longer than 12 h were found dead. The high pressure tolerance of budding yeast is a little weaker than that of tardigrades.