Activation of the intrinsic and extrinsic pathways in high pressure-induced apoptosis of murine erythroleukemia cells

Cellular & Molecular Biology Letters

Shoichiro Tsuruhara¹  Shigeyuki Terada¹  Wakako Iwamoto¹  Satoshi Katsuki¹  Takeo Yamaguchi¹  Kenji Hashiguchi¹ 

[1] Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka, Japan$$

关键词: Apoptosis;    Caspases;    Flow cytometry;    Membrane potential;    High pressure;   

DOI  :  10.2478/s11658-007-0034-x

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

We previously demonstrated that caspase-3, an executioner of apoptosis, is activated in the pressure-induced apoptosis of murine erythroleukemia (MEL) cells (at 100 MPa). Here, we examined the pathway of caspase-3 activation using peptide substrates and caspase inhibitors. Using the substrates of caspases-8 and -9, it was found that both are activated in cells under high pressure. The production of nuclei with sub-G1 DNA content in 100 MPa-treated MEL cells was suppressed by inhibitors of caspases-8 and -9, and pan-caspase. In 100 MPa-treated cells, pan-caspase inhibitor partially prevented the cytochrome c release from the mitochondria and the breakdown of mitochondrial membrane potential. These results suggest that the intrinsic and extrinsic pathways are activated in apoptotic signaling during the high pressure-induced death of MEL cells.

【 授权许可】

Unknown