The neuroprotective effect of picroside II via regulating the expression of myelin basic protein after cerebral ischemia injury in rats

BMC Neuroscience

Meizeng Zhang¹  Xiaojun Ji¹  Yunliang Guo¹  Li Zhao¹ 

[1] Institute of Cerebrovascular Diseases, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, China

关键词: Rats;    MBP;    Cerebral ischemia;    Time window;    Therapeutic dose;    Picroside II;   

Others  :  1092442
DOI  :  10.1186/1471-2202-15-25

 received in 2013-09-04, accepted in 2014-02-11,  发布年份 2014

【 摘 要 】

Background

To explore the neuroprotective effect and optimize the therapeutic dose and time window of picroside II by orthogonal test and the expression of myelin basic protein (MBP) in cerebral ischemic injury in rats. Bilateral common carotid artery occlusion (BCCAO) was used to establish forebrain ischemia models. The successful rat models were grouped according to orthogonal experimental design and injected picroside II intraperitoneally at different ischemic time with different doses. Myelin sheath fast green staining(FGS) and transmission electron microscopy (TEM) were used to observe nerve fiber myelin; the expression of MBP was tested qualitatively and quantitatively by immunohistochemical assay (IHC) and Western blot (WB); Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the transcription level of MBP mRNA.

Results

The protective effect of picroside II was presented by increasing the expression of MBP and decreasing demyelination after cerebral ischemic injury. The best therapeutic time window and dose was (1) ischemia 2.0 h with picroside II 10 mg/kg body weight according to the results of FGS, IHC and WB; (2) ischemia 1.5 h with picroside II 20 mg/kg according to the analysis of RT-PCR.

Conclusion

Given the principle of the longest time window and the lowest therapeutic dose, the optimized therapeutic dose and time window should be injecting picroside II intraperitoneally with 10-20 mg/kg body weight at ischemia 1.5-2.0 h in cerebral ischemic injury.

【 授权许可】

   
2014 Zhao et al.; licensee BioMed Central Ltd.

【 图 表 】

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