Hepadnavirus detected in bile and liver samples from domestic pigs of commercial abattoirs

BMC Microbiology

Vanessa Salete de Paula³  Marcelo Alves Pinto³  Livia Melo Villar⁴  Marcia Arissawa¹  Carlos Eduardo Pereira Velloso³  Moyra Machado Portilho⁴  Debora Regina Lopes dos Santos²  Yasmine Rangel Vieira³ 

[1] Laboratório de Tecnologia de Anticorpos Monoclonais, Bio-Manguinhos, FIOCRUZ, Rio de Janeiro, RJ, Brasil;Departamento de Mircobiologia Veterinária e Imunologia, Universidade Federal Rural do Rio de Janeiro, UFRRJ, Seropédica, RJ, Brasil;Laboratório de Desenvolvimento Tecnológico em Virologia, Pavilhão Hélio e Peggy Pereira – 2° andar - sala B220, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil, n° 4365, Manguinhos - RJ, Rio de Janeiro, Cep: 21045-900, RJ, Brasil;Laboratório de Hepatites Virais, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brasil

关键词: Immunofluorescence;    Genotyping;    Liver;    Bile;    Commercial swine;    Brazil;    Hepadnavirus;   

Others  :  1090825
DOI  :  10.1186/s12866-014-0315-2

 received in 2014-06-21, accepted in 2014-11-26,  发布年份 2014

【 摘 要 】

Background

Preliminary studies showed the prevalence of a virus similar to human hepatitis B virus (HBV-like) in swine from farms in China and the molecular evidence of Hepadnavirus infection in domestic pigs herds in Brazil. In this study, we genetically characterize the swine Hepadnavirus strains in swine from slaughterhouses located in certified abattoirs from Rio de Janeiro State, Brazil and evaluate its hepatotropic potential.

Results

Bile and liver samples from swine were positive for partial genome amplification (ORF S and ORF C), direct sequencing and viral load quantification. Sequencing of the gene encoding the surface antigen allowed classification of Hepadnavirus into genotypes, similar to HBV genotype classification. Indirect immunofluorescence confirmed the presence of HBsAg antigen in liver tissue sections.

Conclusions

So far our data suggest that commercial swine house an HBV-like virus and this relevant finding should be considered in studies on the origin and viral evolution.

【 授权许可】

   
2014 Vieira et al.; licensee BioMed Central Ltd.

【 图 表 】

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