Antioxidant activity of Coriandrum sativum and protection against DNA damage and cancer cell migration

BMC Complementary and Alternative Medicine

MS Kanthimathi¹  Shin Yee Fung¹  Jayakumar Rajarajeswaran¹  Esther LH Tang¹ 

[1]Department of Molecular Medicine, UMCPR (University of Malaya Centre for Proteomics Research), Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia

关键词: Comet assay;    Cancer cell migration;    DNA damage;    Cell cycle;    Caspase;    Hydrogen peroxide;    MCF-7;    Anticancer activity;    Antioxidants;    Coriandrum sativum;   

Others  :  1220459
DOI  :  10.1186/1472-6882-13-347

 received in 2013-07-02, accepted in 2013-12-05,  发布年份 2013

【 摘 要 】

Background

Coriandrum sativum is a popular culinary and medicinal herb of the Apiaceae family. Health promoting properties of this herb have been reported in pharmacognostical, phytochemical and pharmacological studies. However, studies on C. sativum have always focused on the aerial parts of the herb and scientific investigation on the root is limited. The aim of this research was to investigate the antioxidant and anticancer activities of C. sativum root, leaf and stem, including its effect on cancer cell migration, and its protection against DNA damage, with special focus on the roots.

Methods

Powdered roots, leaves and stems of C. sativum were extracted through sequential extraction using hexane, dichloromethane, ethyl acetate, methanol and water. Total phenolic content, FRAP and DPPH radical scavenging activities were measured. Anti-proliferative activitiy on the breast cancer cell line, MCF-7, was assayed using the MTT assay. Activities of the antioxidant enzymes, catalase, superoxide dismutase, glutathione peroxidase, and of the caspases-3, -8 and -9 were assayed on treatment with the extract. Cell cycle progression was analysed using flow cytometry. The scratch motility assay was used to assess inhibition of MCF-7 cell migration. DNA damage in 3 T3-L1 fibroblasts was evaluated by the comet assay. The components in the extract were identified by HPLC and GC-MS.

Results

The ethyl acetate extract of C. sativum roots showed the highest antiproliferative activity on MCF-7 cells (IC₅₀ = 200.0 ± 2.6 μg/mL) and had the highest phenolic content, FRAP and DPPH scavenging activities among the extracts. C. sativum root inhibited DNA damage and prevented MCF-7 cell migration induced by H₂O₂, suggesting its potential in cancer prevention and inhibition of metastasis. The extract exhibited anticancer activity in MCF-7 cells by affecting antioxidant enzymes possibly leading to H₂O₂ accumulation, cell cycle arrest at the G₂/M phase and apoptotic cell death by the death receptor and mitochondrial apoptotic pathways.

Conclusions

This study is the first report on the antioxidant and anticancer properties of C. sativum root. The herb shows potential in preventing oxidative stress-related diseases and would be useful as supplements used in combination with conventional drugs to enhance the treatment of diseases such as cancer.

【 授权许可】

   
2013 Tang et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]DeSylvia D, Stuber M, Fung CC, Bazargan-Hejazi S, Cooper E: The knowledge, attitudes and usage of complementary and alternative medicine of medical students. Evid-Based Compl Alt 2011. 10.1093/ecam/nen075
• [2]Benz CC, Yau C: Ageing, oxidative stress and cancer: paradigms in parallax. Nat Rev Cancer 2008, 8:875-879.
• [3]Jiménez-Estrada M, Velázquez-Contreras C, Garibay-Escobar A, Sierras-Canchola D, Lapizco-Vázquez R, Ortiz-Sandoval C, Burgos-Hernández A, Robles-Zepeda RE: In vitro antioxidant and antiproliferative activities of plants of the ethnopharmacopeia from northwest of Mexico. BMC Complem Altern M 2013, 13:12. BioMed Central Full Text
• [4]Imlay JA, Linn S: DNA damage and oxygen radical toxicity. Science 1988, 240:1302.
• [5]Nelson KK, Ranganathan AC, Mansouri J, Rodriguez AM, Providence KM, Rutter JL, Pumiglia K, Bennett JA, Melendez JA: Elevated sod2 activity augments matrix metalloproteinase expression evidence for the involvement of endogenous hydrogen peroxide in regulating metastasis. Clin Cancer Res 2003, 9:424-432.
• [6]López-Lázaro M: Dual role of hydrogen peroxide in cancer: possible relevance to cancer chemoprevention and therapy. Cancer Lett 2007, 252:1-8.
• [7]Taraphdar AK, Roy M, Bhattacharya R: Natural products as inducers of apoptosis: implication for cancer therapy and prevention. Curr Sci India 2001, 80:1387-1396.
• [8]Sreelatha S, Padma P, Umadevi M: Protective effects of Coriandrum sativum extracts on carbon tetrachloride-induced hepatotoxicity in rats. Food Chem Toxicol 2009, 47:702-708.
• [9]Padmaa M: Coriandrum sativum linn: a review. Pharmacol online Newsl 2009, 3:561-573.
• [10]Asgarpanah J, Kazemivash N: Phytochemistry, pharmacology and medicinal properties of Coriandrum sativum L. Afr J Pharm Pharmaco 2012, 6:2340-2345.
• [11]Oganesyan E, Nersesyan Z, Parkhomenko AY: Chemical composition of the above-ground part of Coriandrum sativum. Pharm Chem J-Ussr 2007, 41:149-153.
• [12]Kubo I, Fujita K-i, Kubo A, Nihei K-i, Ogura T: Antibacterial activity of coriander volatile compounds against Salmonella choleraesuis. J Agr Food Chem 2004, 52:3329-3332.
• [13]Momin AH, Acharya SS, Gajjar AV: Coriandrum sativum-review of advances in phytopharmacology. Int J Pharm Sci Res 2012, 3:1233-1239.
• [14]Wangensteen H, Samuelsen AB, Malterud KE: Antioxidant activity in extracts from coriander. Food Chem 2004, 88:293-297.
• [15]Hashim M, Lincy S, Remya V, Teena M, Anila L: Effect of polyphenolic compounds from Coriandrum sativum on H2O2-induced oxidative stress in human lymphocytes. Food Chem 2005, 92:653-660.
• [16]Singleton V, Rossi JA: Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 1965, 16:144-158.
• [17]Benzie IFF, Strain J: The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 1996, 239:70-76.
• [18]Cos P, Rajan P, Vedernikova I, Calomme M, Pieters L, Vlietinck AJ, Augustyns K, Haemers A, Berghe DV: In vitro antioxidant profile of phenolic acid derivatives. Free Radical Res 2002, 36:711-716.
• [19]Mosmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983, 65:55-63.
• [20]Sato Y, Rifkin DB: Autocrine activities of basic fibroblast growth factor: regulation of endothelial cell movement, plasminogen activator synthesis, and DNA synthesis. J Cell Biol 1988, 107:1199-1205.
• [21]Singh NP, McCoy MT, Tice RR, Schneider EL: A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988, 175:184-191.
• [22]Jayakumar R, Kanthimathi MS: Dietary spices protect against hydrogen peroxide-induced DNA damage and inhibit nicotine-induced cancer cell migration. Food Chem 2012, 134:1580-1584.
• [23]Nuutila A, Kammiovirta K, Oksman-Caldentey K-M: Comparison of methods for the hydrolysis of flavonoids and phenolic acids from onion and spinach for HPLC analysis. Food Chem 2002, 76:519-525.
• [24]Conde E, Moure A, Domínguez H, Parajó JC: Fractionation of antioxidants from autohydrolysis of barley husks. J Agr Food Chem 2008, 56:10651-10659.
• [25]Moirangthem DS, Talukdar NC, Kasoju N, Bora U: Antioxidant, antibacterial, cytotoxic, and apoptotic activity of stem bark extracts of Cephalotaxus griffithii Hook. f. BMC Complem Altern M 2012, 12:30. BioMed Central Full Text
• [26]de Almeida ME, Mancini Filho J, Barbosa Guerra N: Characterization of antioxidant compounds in aqueous coriander extract (Coriandrum sativum L.). LWT-Food Sci Technol 2005, 38:15-19.
• [27]Ford J, Jiang M, Milner J: Cancer-specific functions of SIRT1 enable human epithelial cancer cell growth and survival. Cancer Res 2005, 65:10457-10463.
• [28]Min JY, Lim SO, Jung G: Downregulation of catalase by reactive oxygen species via hypermethylation of CpG island II on the catalase promoter. FEBS Lett 2010, 584:2427-2432.
• [29]Pigeolet E, Remacle J: Susceptibility of glutathione peroxidase to proteolysis after oxidative alteration by peroxides and hydroxyl radicals. Free Radical Bio Med 1991, 11:191-195.
• [30]Rashad AE, El-Sayed WA, Mohamed AM, Ali MM: Synthesis of new quinoline derivatives as inhibitors of human tumor cells growth. Arch Pharm 2010, 343:440-448.
• [31]Evens AM, Lecane P, Magda D, Prachand S, Singhal S, Nelson J, Miller RA, Gartenhaus RB, Gordon LI: Motexafin gadolinium generates reactive oxygen species and induces apoptosis in sensitive and highly resistant multiple myeloma cells. Blood 2005, 105:1265-1273.
• [32]Burdock GA, Carabin IG: Safety assessment of coriander (Coriandrum sativum L.) essential oil as a food ingredient. Food Chem Toxicol 2009, 47:22-34.
• [33]Fan TJ, Han LH, Cong RS, Liang J: Caspase family proteases and apoptosis. Acta Bioch Bioph Sin 2005, 37:719-727.
• [34]Day TW, Huang S, Safa AR: c-FLIP knockdown induces ligand-independent DR5-, FADD-, caspase-8-, and caspase-9-dependent apoptosis in breast cancer cells. Biochem Pharmacol 2008, 76:1694-1704.
• [35]Riedl SJ, Shi Y: Molecular mechanisms of caspase regulation during apoptosis. Nat Rev Mol Cell Bio 2004, 5:897-907.
• [36]Jänicke RU: MCF-7 breast carcinoma cells do not express caspase-3. Breast Cancer Res Tr 2009, 117:219-221.
• [37]Zhang GP, Lu YY, Lv JC, Ou HJ: Effect of ursolic acid on caspase-3 and PARP expression of human MCF-7 cells. Zhongguo Zhong Yao Za Zhi 2006, 31:141-144.
• [38]Yang HL, Chen CS, Chang WH, Lu FJ, Lai YC, Chen CC, Hseu TH, Kuo CT, Hseu YC: Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by Antrodia camphorata. Cancer Lett 2006, 231:215-227.
• [39]Gooch JL, Yee D: Strain-specific differences in formation of apoptotic DNA ladders in MCF-7 breast cancer cells. Cancer Lett 1999, 144:31-37.
• [40]Shim HY, Park JH, Paik HD, Nah SY, Kim DS, Han YS: Acacetin-induced apoptosis of human breast cancer MCF-7 cells involves caspase cascade, mitochondria-mediated death signaling and SAPK/JNK1/2-c-Jun activation. Mol Cells 2007, 24:95-104.
• [41]Schwartz GK, Shah MA: Targeting the cell cycle: a new approach to cancer therapy. J Clin Oncol 2005, 23:9408-9421.
• [42]Cortés-Eslava J, Gómez-Arroyo S, Villalobos-Pietrini R, Espinosa-Aguirre JJ: Antimutagenicity of coriander (Coriandrum sativum) juice on the mutagenesis produced by plant metabolites of aromatic amines. Toxicol Lett 2004, 153:283-292.
• [43]Robbins RJ: Phenolic acids in foods: an overview of analytical methodology. J Agr Food Chem 2003, 51:2866-2887.
• [44]Ng LK, Lafontaine P, Harnois J: Gas chromatographic–mass spectrometric analysis of acids and phenols in distilled alcohol beverages: application of anion-exchange disk extraction combined with in-vial elution and silylation. J Chromatogr A 2000, 873:29-38.
• [45]Dias MI, Barros L, Sousa MJ, Ferreira IC: Comparative study of lipophilic and hydrophilic antioxidants from in vivo and in vitro grown Coriandrum sativum. Plant Food Hum Nutr 2011, 66:181-186.
• [46]Taniguchi M, Yanai M, Xiao YQ, Kido T-i, Baba K: Three isocoumarins from Coriandrum sativum. Phytochemistry 1996, 42:843-846.
• [47]Esiyok D, Otles S, Akcicek E: Herbs as a food source in Turkey. Asian Pac J Cancer P 2004, 5:334-339.
• [48]Lei J, Yu J, Yu H, Liao Z: Composition, cytotoxicity and antimicrobial activity of essential oil from Dictamnus dasycarpus. Food Chem 2008, 107:1205-1209.
• [49]Pandey A, Bigoniya P, Raj V, Patel K: Pharmacological screening of Coriandrum sativum Linn. for hepatoprotective activity. J Pharm Bioallied Sci 2011, 3:435.
• [50]Chung I-M, Ahmad A, Kim S-J, Naik PM, Nagella P: Composition of the essential oil constituents from leaves and stems of Korean Coriandrum sativum and their immunotoxicity activity on the Aedes aegypti L. Immunopharm Immunot 2012, 34:152-156.
• [51]Putchala MC, Ramani P, Sherlin HJ, Premkumar P, Natesan A: Ascorbic acid and its pro-oxidant activity as a therapy for tumours of oral cavity–a systematic review. Arch Oral Biol 2013, 58:563-574.
• [52]Kurbacher CM, Wagner U, Kolster B, Andreotti PE, Krebs D, Bruckner HW: Ascorbic acid (vitamin C) improves the antineoplastic activity of doxorubicin, cisplatin, and paclitaxel in human breast carcinoma cells in vitro. Cancer Lett 1996, 103:183-189.
• [53]Osmak M, Kovacek I, Ljubenkov I, Spaventi R, Eckert-Maksic M: Ascorbic acid and 6-deoxy-6-chloro-ascorbic acid: potential anticancer drugs. Neoplasma 1997, 44:101-108.
• [54]Chen Q, Espey MG, Krishna MC, Mitchell JB, Corpe CP, Buettner GR, Shacter E, Levine M: Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissues. Proc Natl Acad Sci USA 2005, 102:13604-13609.
• [55]Matés JM, Sánchez-Jiménez FM: Role of reactive oxygen species in apoptosis: implications for cancer therapy. Int J Biochem Cell B 2000, 32:157-170.
• [56]Bijur GN, Briggs B, Hitchcock CL, Williams MV: Ascorbic acid-dehydroascorbate induces cell cycle arrest at G2/M DNA damage checkpoint during oxidative stress. Environ Mol Mutagen 1999, 33:144-152.
• [57]Cha J, Roomi MW, Ivanov V, Kalinovsky T, Niedzwiecki A, Rath M: Ascorbate supplementation inhibits growth and metastasis of B16FO melanoma and 4 T1 breast cancer cells in vitamin C-deficient mice. Int J Oncol 2013, 42:55.