Green Chemistry Glucose Biosensor Development using Etlingera elatior Extract

International Conference on Chemistry and Material Science 2017

化学;材料科学

Fatoni, A.^1,2 ; Anggraeni, M.D.^3 ; Zusfahair^1 ; Iqlima, H.^1

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Purwokerto

53123, Indonesia^1

Center for Maritime Biosciences Studies, Universitas Jenderal Soedirman, Purwokerto, Jawa Tengah

53123, Indonesia^2

Department of Nursing, Faculty of Health Sciences, Universitas Jenderal Soedirman, Purwokerto, Indonesia^3

关键词: Analysis method;    Diabetes mellitus;    Etlingera elatior;    Glucose biosensor;    Glucose concentration;    Heating treatments;    Regression equation;    Wide-linear range;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/299/1/012015/pdf
DOI  :  10.1088/1757-899X/299/1/012015

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Glucose biosensor development is one of the important strategies for early detection of diabetes mellitus disease. This study was aimed to explore the flower extract of Etlingera elatior for a green-analysis method of glucose biosensor. Flowers were extracted using ethanol: HCl and tested its performances as an indicator of glucose biosensor using glucose oxidase enzyme. The glucose oxidase react with glucose resulted hydrogen peroxide that would change the color of the flower extract. Furthermore, the extract was also studied including their stability to pH, oxidizing and reducing, temperature, and storage. The results showed that the Etlingera elatior extract had high correlation between color change and glucose concentration with regression equation of y = -0.0005x + 0.4724 and R2of 0.9965. The studied biosensor showed a wide linear range to detect glucose sample of 0 to 500 mM. The extract characterization showed a more stable in low pH (acid), reducing agent addition, heating treatment and storage.