Alginate cryogel based glucose biosensor

10th Joint Conference on Chemistry

Fatoni, Amin^1 ; Dwiasi, Dian Windy^1 ; Hermawan, Dadan^1

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Jl. Dr. Soeparno, Purwokerto

53123, Indonesia^1

关键词: Biomolecule immobilization;    Glucose biosensor;    Large surface area;    Macroporous structures;    Operational stability;    Porous structures;    Potassium permanganate;    Sub-zero temperatures;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/107/1/012010/pdf
DOI  :  10.1088/1757-899X/107/1/012010

来源: IOP

【 摘 要 】

Cryogel is macroporous structure provides a large surface area for biomolecule immobilization. In this work, an alginate cryogel based biosensor was developed to detect glucose. The cryogel was prepared using alginate cross-linked by calcium chloride under sub-zero temperature. This porous structure was growth in a 100 μL micropipette tip with a glucose oxidase enzyme entrapped inside the cryogel. The glucose detection was based on the colour change of redox indicator, potassium permanganate, by the hydrogen peroxide resulted from the conversion of glucose. The result showed a porous structure of alginate cryogel with pores diameter of 20-50 μm. The developed glucose biosensor was showed a linear response in the glucose detection from 1.0 to 5.0 mM with a regression of y = 0.01x+0.02 and R2of 0.994. Furthermore, the glucose biosensor was showed a high operational stability up to 10 times of uninterrupted glucose detections.