Effect of centrifugation at 7,000 g, 8,000 g, and 9,000 g on the salivary protein profile ≥30 kDa

1st Physics and Technologies in Medicine and Dentistry Symposium

物理学;医药卫生

Baskoro, B.D.^1 ; Nugraha, R.A.^1 ; Puspitawati, R.^1 ; Redjeki, S.^1

Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia^1

关键词: Operational procedures;    Protein profiles;    Protein separations;    Salivary proteins;    SDS PAGE;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/884/1/012013/pdf
DOI  :  10.1088/1742-6596/884/1/012013

学科分类：卫生学

来源: IOP

【 摘 要 】

There are no established standard operational procedures of centrifugation for protein separation. Centrifugation at 10,000 g separates ≥30 kDa salivary proteins. The aim of this study is to determine the effect of centrifugation at 7,000 g, 8,000 g, and 9,000 g on the salivary protein profile and the frequency of ≥30 kDa salivary proteins. Post-centrifuged salivary supernatant was analyzed using SDS-PAGE. An increased centrifugation speed resulted in the decreased frequency of ≥30 kDa salivary protein emergence. Each centrifugation speed resulted in a different protein profile. Centrifugation at 7,000 g, 8,000 g, and 9,000 g influences the frequency of salivary protein emergence and protein profiles ≥30 kDa.