Antioxidant Effect of Clerodendrum sp and Acanthus illicifolius Methanol Extraction on Blood Profile of Male Mice Induced by Benzo(α)pyrene

4th International Conference on Biological Sciences and Biotechnology

Widiastuti, Endang Linirin^1^2 ; Arifianti, Rizka^1 ; Khairani, Iffa A.^1 ; Christianto, Y.^1 ; Ara, Naufallia F.^1 ; Maharani, Henni W.^2

Biology Department, Faculty Math and Sciences, University of Lampung, Indonesia^1

Center of Coastal and Marine Sciences, University of Lampung, Jl. Prof. Dr. Sumantribrojonegoro No. 1, Bandar Lampung, Lampung

35145, Indonesia^2

关键词: Animal studies;    Antioxidant effect;    Completely randomized designs;    Light cycles;    Mus musculus;    One-way anova;    Protein profiles;    Treatment group;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/305/1/012011/pdf
DOI  :  10.1088/1755-1315/305/1/012011

来源: IOP

【 摘 要 】

Benzo(α)pyrene is one of carcinogenic PAH substances which commonly produced by many different sources of polutant. The study was conducted in order to determine the effect of methanol extraction of Clerodendrum sp and Acanthus illicifolius which known to be two of common mangrove plants on the blood profile of male mice induced by benzo(α)pyrene (one of polycyclic aromatic hydrocarbon/PAH). Thirty male mice (Mus musculus) of ddy strain from Lampung Vetenary Agency were used to conduct the study. Completely randomized design was assigned for this study. Mice were grouped into 5 different group treated with PAH injection, one groups used as control without any injection, one as postitif group with PAH injection but not given with any methanol extraction and the last three group were given with methanol extraction of Clerodendrum and Acanthus and taurine prior PAH injection. All the animal study were kept in room temperature with 12:12 light cycle and fed and water ad libitum. Observation of their blood cells and protein profile was made after 14 days. Data was analyzed by One-Way Anova at 5% followed by Fisher's at level of p