Hyaluronic Acid (HA)-Polyethylene glycol (PEG) as injectable hydrogel for intervertebral disc degeneration patients therapy

International Conference on Physical Instrumentation and Advanced Materials

物理学;材料科学

Kwarta, Cityta Putri^1 ; Widiyanti, Prihartini^2 ; Siswanto^3

Biomedical Engineering Program, Faculty of Science and Technology, Universitas Airlangga, Indonesia^1

Institute of Tropical Diseases, Universitas Airlangga, Indonesia^2

Physics Department, Faculty of Science and Technology, Universitas Airlangga, Indonesia^3

关键词: Characteristic properties;    Chronic low back pain;    Cytotoxicity assays;    Enzyme concentrations;    Injectable hydrogels;    Inter-vertebral disc degeneration;    Minimally invasive surgery;    Polymer composite;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/853/1/012036/pdf
DOI  :  10.1088/1742-6596/853/1/012036

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Chronic Low Back Pain (CLBP) is one health problem that is often encountered in a community. Inject-able hydrogels are the newest way to restore the disc thickness and hydration caused by disc degeneration by means of minimally invasive surgery. Thus, polymers can be combined to improve the characteristic properties of inject-able hydrogels, leading to use of Hyaluronic Acid (a natural polymer) and Polyethylene glycol (PEG) with Horse Radish Peroxide (HRP) cross linker enzymes. The swelling test results, which approaches were the ideal disc values, were sampled with variation of enzyme concentrations of 0.25 μmol/min/mL. The enzyme concentrations were 33.95%. The degradation test proved that the sample degradation increased along with the decrease of the HRP enzyme concentration. The results of the cytotoxicity assay with MTT assay method showed that all samples resulted in the 90% of living cells are not toxic. In vitro injection, models demonstrated that higher concentration of the enzymes was less state of gel which would rupture when released from the agarose gel. The functional group characterization shows the cross linking bonding in sample with enzyme adding. The conclusion of this study is PEG-HA-HRP enzyme are safe polymer composites which have a potential to be applied as an injectable hydrogel for intervertebral disc degeneration.