An indole alkaloid produced by Indonesian's marine sponge Raspailia ramosa as an inhibitory of the panc-1 cell adapted to nutrient starvation

International Conference on Fisheries, Aquatic, and Environmental Sciences 2018

地球科学;农业科学;生态环境科学

Kurnianda, V.^1,2,3 ; Ramadhan, M.R.^1 ; Karina, S.^1 ; Agustina, S.^1 ; Ulfah, M.^1 ; Octavina, C.^1 ; Mardiah, A.^1 ; Syahliza, F.^1 ; Purnawan, S.^1

Department of Marine Science, Faculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh

23111, Indonesia^1

Marine Chemistry Laboratory, Faculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh

23111, Indonesia^2

Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa

903-0213, Japan^3

关键词: Active metabolites;    Bioactive compounds;    Cytotoxic activities;    Indole alkaloids;    Molecular formula;    Nutrient-starvation;    Potential activities;    Secondary amines;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/216/1/012042/pdf
DOI  :  10.1088/1755-1315/216/1/012042

来源: IOP

【 摘 要 】

The bioactivity from Indonesian's Marine Sponge Raspailia ramosa has been done on July 2018. Bioactive compound isolated based on bioassay-guided separation with several steps of chromatography. The active metabolite compound of Raspailia ramosa has cytotoxic activity against PANC-1 cell with IC50 = 10g/mL. Based on NMR spectrum shows that the active compound has molecular formula C16H17N4Br determined by LCMS-ESI with molecular weight [M+H]+345.23 m/z. The FTIR spectrum shows that metabolic metabolites have NH secondary amines 3435.56 cm-1 with CN imine fingerprint area (1637.27 cm-1), CH methyl (2853.39 cm-1), CH methylene (2769.64 cm-1) indicate as indole alkaloid. The active metabolite compound of Raspailia ramosa has cytotoxic activity against PANC-1 cellwithIC50 = 10 g / mL. This compound has the potential activity for the drug model for commercial drug antimycin = 0.1 μg / mL.