Quantity and quality of guinea pig (cavia porcellus) spermatozoa after administration of methanol extract of bitter melon (momordica charantia) seed and depot medroxy progesterone acetate (DMPA)

3rd International Conference on Biological Sciences and Biotechnology

Ilyas, Syafruddin^1,2 ; Hutahaean, Salomo^1 ; Nursal^1

Biology Departement, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Kampus USU, Jl. Bioteknologi No. 1, Padang Bulan, Medan, Indonesia^1

Featured Center for Science and Technology Stem Cells, Universitas Sumatera Utara, Medan, Kampus USU Padang Bulan, Jalan Dr. Masyur No. 4, Medan, Indonesia^2

关键词: antifertility;    Dimethyl sulfoxide (DMSO);    Experimental animals;    Experimental methods;    Guinea pigs;    Methanol extract;    Momordica charantia;    Randomized design;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012047/pdf
DOI  :  10.1088/1755-1315/130/1/012047

来源: IOP

【 摘 要 】

The discovery of male contraceptive drugs continues to be pursued, due to the few participation of men associated with the lack of contraceptive options for men. The combination of bitter melon seed methanol extract and DMPA are the options that currently apply to men. Therefore, the use of guinea pigs as experimental animals conducted research using experimental methods with complete randomized design (CRD). There are 4 control groups and 4 treatment groups. The first group, control group of dimethyl sulphoxide (DMSO) for 0 week (K0), The second one, bitter melon seed extract of 50 mg/100g Body Weight/day for 0 week (P0), the third one, control group of dimethyl sulfoxide (DMSO) for 4 weeks (K1), the fourth one, bitter melon seed extract of 50 mg/100g BW/day for 4 weeks + Depot medroxy Progesterone Acetate (P1), the fifth one, control group of dimethyl sulfoxide (DMSO) for 8 weeks (K2), the sixth one, bitter melon seed extract of 50 mg/100g BW/day for 8 weeks + DMPA (P2), the seventh one, control group of dimethyl sulfoxide (DMSO) for 12 weeks (K3), the eighth one, bitter melon seed extract of 50 mg/100g BW/day for 12 weeks + DMPA (P3). Methanol extract of bitter melon seed to decrease the quantity and quality of guinea pig spermatozoa decreased significantly, i.e. viability and normal morphology of spermatozoa (p