Lipase production from Bacillus subtilis with submerged fermentation using waste cooking oil

2nd international Tropical Renewable Energy Conference 2017

Suci, M.^1 ; Arbianti, R.^1 ; Hermansyah, H.^1

Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok West Java

16424, Indonesia^1

关键词: Bacillus Subtilis;    Hydrolysis reaction;    Lipase production;    Lipolytic activity;    Nitrogen sources;    Submerged fermentation;    Waste cooking oil;    Yeast extracts;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/105/1/012126/pdf
DOI  :  10.1088/1755-1315/105/1/012126

来源: IOP

【 摘 要 】

Bacterial lipase has been developed lately because of its advantage to produce with large scale. Culture of Bacillus subtilis was grown to produce lipase in Waste Cooking Oil (WCO) using submerged fermentation (SmF) method. The enzyme activity of the culture was improved by using different concentration of inoculum, substrate, nitrogen source, inducer, and Ca2+ion at 30°C for 84h fermentation. Lipolytic activity of crude lipase was determined using titrimetric method with hydrolysis reaction. Maximum activity of lipase (4.96 U/mL) was found at 5% (v/v) inoculum, 4% (v/v) WCO, 0.5% (w/v) yeast extract, 0.25% (v/v) olive oil, and 10 mM Ca2+that present in medium culture. Later, the crude lipase has been dried with spray dryer and resulting 17.33 gr of dry lipase powder per 500 mL crude lipase.