Thermal and Reused Stability of Immobilized Lipase in Carrageenan

International Conference on Food Science and Technology

Wardoyo, F.A.^1 ; Hidayah, F.F.^2

Department of Medical Laboratory Technology, University of Muhammadiyah Semarang, Central Java, Indonesia^1

Department of Chemistry Education, University of Muhammadiyah Semarang, Central Java, Indonesia^2

关键词: Carrageenans;    Catalytic ability;    Entrapment method;    High temperature;    Hydrolysis reaction;    Immobilized lipase;    Reaction cycles;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/292/1/012028/pdf
DOI  :  10.1088/1755-1315/292/1/012028

来源: IOP

【 摘 要 】

Lipase is one kind of enzyme that is widely used as a catalyst in industrial and medical. Lipase has a great catalytic ability, but is easily affected by temperature, and is also difficult to separate at the end of the reaction. This causes most of lipase used only in one reaction cycle. Therefore an enzyme immobilization is needed, so that lipase can maintenance it's activity in high temperatures, and can also be separated at the end of the reaction so that it can be re-used. This study aimed to determine the thermal stability and re-used stability of the immobilized lipase in carrageenan. The method used for immobilizing lipase is through entrapment method. The effectiveness of immobilized lipase tested through the hydrolysis reaction of palm oil. The result was found that the immobilized lipase in carrageenan was able to maintain its catalytic activity up to 50 °C and also up to five reaction cycles.