Oyster mushroom's lipase enzyme entrapment on calcium alginate as biocatalyst in the synthesis of lauryl diethanolamide

11th Joint Conference on Chemistry in Conjunction with the 4th Regional Biomaterials Scientific Meeting

材料科学;化学;生物科学

Wijayati, N.^1 ; Masubah, K.^1 ; Supartono^1

Department of Chemistry, Semarang State University, Indonesia^1

关键词: Biotechnology applications;    Calcium alginate;    Entrapment method;    Food industries;    Lipase immobilization;    Optimum conditions;    Oyster mushroom;    Protein contents;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/172/1/012034/pdf
DOI  :  10.1088/1757-899X/172/1/012034

来源: IOP

【 摘 要 】

Lipase is an enzyme with large biotechnology applications, such as hydrolysis in the food industry, applications in chemical industry, synthesis of polymers and surfactants. Lipase was isolated from oyster mushroom with activity 0,93 U/mg and protein content 1,1234 mg/mL. Lipase was immobilized by entrapment method in a matrix of Ca-alginate. This report describes that we have developed for the synthesis of lauryl diethanolamide The result showed that the optimum condition of lipase immobilization was achieved on 3% Na-alginate solution with protein content 0,84 mg/mL and the activity 3,33 U/mg. An amide (22.911%) formed from the amidation of lauric acid and diethanolamine.