Investigation of lipolytic activity of the red king crab hepatopancreas homogenate by NMR spectroscopy

PeerJ

article

Maria Timchenko¹  Vladislav Molchanov²  Maxim Molchanov¹  Alexander Timchenko³  Evgeny Sogorin⁴ 

[1] Laboratory of NMR biosystems, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences;Medical Physics Department, Dubna State University, Branch Protvino;Group of Experimental Research and Engineering Oligomeric Structures, Institute of Protein Research, Russian Academy of Sciences;Institute for Biological Instrumentation, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

关键词: Lipolytic activity;    Paralithodes camtschaticus;    Hepatopancreas homogenate;    NMR spectroscopy;   

DOI  :  10.7717/peerj.12742

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The digestive gland of craboids (hepatopancreas) is rich in a huge number of various enzymes (collagenases, nucleases, hyaluronidases, proteases), which are well studied at the moment. However, little is known about crustacean lipases. In this work, using 1H NMR spectroscopy, it was found that the hepatopancreas homogenate of the red king crab Paralithodes camtschaticus demonstrates high lipolytic activity against triacetin in a wide pH range and shows moderate activity against the caprylic/capric triglyceride emulsion. Under the action of the hepatopancreas homogenate, triacylglycerols are converted into 1,2-diacylglycerol, and then into 2-monoacylglycerol and 1-monoacylglycerol. The 1-monoacylglycerol predominates in the reaction products. The use of NMR spectroscopy makes it possible to quickly detect hydrolysis products and evaluate the reaction direction.

【 授权许可】

CC BY