Immunostimulatory Activity from Pirdot Leaves Ethanolic Extract (Saurauia vulcani Korth.) in Rats (Rattus norvegicus)

4th International Conference on Biological Sciences and Biotechnology

Sinaga, Erlintan^1 ; Ilyas, Syafruddin^2 ; Hutahaean, Salomo^2 ; Sitorus, Panal^3

Department of Educational Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, Medan, Indonesia^1

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia^2

Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia^3

关键词: Ethanol extract;    Ethanolic extracts;    Histological images;    Immunostimulatory activities;    Randomized design;    Sheep red blood cells;    Significance levels;    Spleen histologies;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/305/1/012082/pdf
DOI  :  10.1088/1755-1315/305/1/012082

来源: IOP

【 摘 要 】

Erythrocyte, lymphocyte quantities and spleen histology were observed to investigate the immunostimulatory effect Pirdor leaves ethanolic extract (Saurauia vulcani Korth.) (EES) in white rats (Rattus norvegicus L.) -induced with sheep red blood cell (SRBC) as antigen. Experimental design used in this study was Complete Randomized Design. Twenty four rats were classified into four groups: a control group fed with distilled water (G0), a group treated with sheep red blood cell (SRBC) (G1), a group treated with ethanol extract of pirdot leaves (G2), and a group treated with ethanol extract of pirdot leaves + SRBC (G3) for 31 days. Blood was withrawnThen the red blood cell in white rats were sized by using ABX micros 60. Spleen of white rats were taken and stained by using haematoxylin and eosin to visualize lymphoid follicles. Data were analyzed by one-way ANOVA at 5% significance level. Significant differences were observed in total erythrocytes and spleen histological image which showed a prospect of EES as immunostimulant.