Comparative study of optical coherence tomograph and histological images of eustachian tube nasopharyngeal region and adjacent structures in vivo and ex-vivo miniature pigs

BioMedical Engineering OnLine

Research

Xiao-Mei Sun¹  Qing-Yu Gu¹  Shi-Min Zhuang¹  Guan-Ping Zhang¹  Zhi-Wen Xiao¹  Hui-Qing Zhang¹  Jia-Qi Luo²  Bai-Ling Li³ 

[1] Department of Otolaryngology Head and Neck Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, 510655, Guangzhou, China;Department of Otolaryngology Head and Neck Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, 510655, Guangzhou, China;Department of Otolaryngology, Shenzhen City Baoan District Women’s and Children’s Hospital, Baoan District, 518100, Shenzhen, Guangdong, China;Shenzhen Science & Technology Development Exchange Center, 518000, Shenzhen, China;

关键词: Optical coherence tomograph (OCT);    Histological images;    Miniature pigs;    Eustachian tube;    Nasopharynx;   

DOI  :  10.1186/s12938-023-01104-z

 received in 2022-11-06, accepted in 2023-04-25,  发布年份 2023

来源: Springer

【 摘 要 】

ObjectivesOptical Coherence Tomograph (OCT) imaging technology can be used to examine, in vivo, the human ET. At present, it is impossible to achieve the OCT scanning vivo and ex vivo in the same individual human body, or study the consistency between OCT images and histological images of the eustachian tube nasopharyngeal region and adjacent structures. The aim of this study was to determine the consistency between OCT images and histological sections in vivo and ex vivo in miniature pigs.MethodsOCT imaging was performed on five adult miniature pigs in vivo and ex vivo. The images of the eustachian tube OCT (ET-OCT), nasopharynx OCT (NP-OCT) and histological cross sections were further studied.ResultsAll five miniature pigs achieved the OCT scan successfully, acquiring ET-OCT and NP-OCT images in vivo and ex vivo on both sides. The acquired ET OCT images closely matched the histological images, revealing details of the cartilage, submucosa, glands, and mucosa. The lower segment of the ET wall mucosa had an abundance of glands and submucosal tissues, with more low-signal areas appearing in the ex vivo images. The NP-OCT images of the nasopharynx matched the details of the mucosa and submucosal tissues. The ex-vivo OCT images showed thicker mucosa and more scattered slightly lower signal areas compared to the vivo OCT images.ConclusionsET-OCT images and NP-OCT images matched the histological structure of eustachian tube nasopharyngeal region structures in miniature pigs both in vivo and ex vivo. OCT images may be sensitive to changes in edema and ischemia status. There is a great potential for morphological assessment of inflammation, edema, injure, mucus gland status.

【 授权许可】

CC BY   
© The Author(s) 2023

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