Establishing Novel Bacteriophage Detection Method Based on Imagining Flow Cytometry

2018 4th International Conference on Environmental Science and Material Application

生态环境科学;材料科学

Yang, Jiayi^1 ; Liu, Sizhang^1^2 ; Sui, Zhiwei^1 ; Wang, Jing^1 ; Niu, Chunyan^1

National Institution of Metrology, Beijing

100029, China^1

Changchun Institution of Biological Products Co., Ltd, Changchun

130012, China^2

关键词: Biological characteristic;    Capsid proteins;    Detection methods;    Detection range;    E.coli cells;    Pathogenic virus;    PhiX174;    Safe operation;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/252/4/042116/pdf
DOI  :  10.1088/1755-1315/252/4/042116

来源: IOP

【 摘 要 】

Bacteriophage ΦX174 specifically infects E.coli cells. It shares the similar biological characteristics with pathogenic virus. Thus, it is widely used as the "model virus" in the evaluation of virus control effect due to its safe operation. In this study, we have established a new bacteriophage detection method based on imagining flow cytometry. Combining the nucleic acid dyeing and capsid protein fluorescence labeling, the method based on imagining flow cytometry shows good linearity and specificity within its detection range. The establishment of this method is of great importance in fast and accurate detection for bacteriophage.