Quantitative detection of Deoxynivalenol in food and feed based on nanogold immunochromatographic assay

4th International Conference on Energy Equipment Science and Engineering

Jin, Yu^1 ; Liu, Renrong^1 ; Zhu, Lixin^1 ; Li, Min^1 ; Lei, Shuijuan^1 ; Qiu, Xiaoyan^1

School of Life Science, Jiangxi Science and Technology Normal University, Nanchang, Jiangxi

330013, China^1

关键词: Coefficient of variation;    Detection methods;    Detection process;    Detection range;    Immunochromatographic;    Immunochromatographic assays;    Linear fitting;    Quantitative detection;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/242/4/042017/pdf
DOI  :  10.1088/1755-1315/242/4/042017

来源: IOP

【 摘 要 】

A deoxynivalenol (DON) monoclonal antibody (DON-Mab) was labeled with gold nanospherical (AuNSs) with a particle size of 45±1.9 nm. The DON Immunochromatographic test card (DON-GICT) for detecting DON in food and feed was developed. The sample does not require special handling. The detection process takes about 20-25 minutes. In conjunction with the nano gold reader, the detection range of the actual sample by DON-GICT is 6.25-6250 μg/kg. DON standards with a final concentration of 500μg/kg were added to 5 grains and 1 feed sample, respectively, and the recovery was 98.3%-127.0%, and the coefficient of variation was 0.9%-4.7%. Twenty-three feed samples and 11 food samples were tested by DON-ELISA and DON-GICT, respectively, and the results were compared. The linear fitting correlation coefficient R2 = 0.9845. It shows that DON-GICT has good accuracy, precision and wide detection range, which can provide efficient and accurate detection method for detecting DON pollution in food and feed.