Determination of the properties and loading efficiency of encapsulated BSA-FITC and dexamethasone for drug delivery systems

16th International Conference of Students and Young Scientists "Prospects of Fundamental Sciences Development"

自然科学（总论）

Chudinova, E.^1 ; Surmeneva, M.^1 ; Koptyug, A.^2 ; Sokolova, V.^3 ; Prymak, O.^3 ; Bouckercha, S.^3 ; Epple, M.^3 ; Surmenev, R.^1

Physical Materials Science and Composite Materials Centre, National Research Tomsk Polytechnic University, 30 Lenin Avenue, Tomsk

634050, Russia^1

Department of Mechanical Engineering and Quality Technology, SportsTech Research Centre, Mid Sweden University, Akademigatan 1, Östersund

SE 831 25, Sweden^2

Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen

45117, Germany^3

关键词: Bovine serum albumins;    Drug delivery system;    Encapsulated drugs;    Fluorescein isothiocyanate;    Hydrodynamic diameter;    Layer-by-layer assemblies;    Polymer microcapsules;    Visible spectroscopy;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/597/1/012056/pdf
DOI  :  10.1088/1757-899X/597/1/012056

学科分类：自然科学（综合）

来源: IOP

【 摘 要 】

In this work porous microparticles of calcium carbonate were synthesized with bovine serum albumin - fluorescein isothiocyanate conjugate (BSA-FITC) and dexamethasone, and then used for encapsulation in polymer microcapsules by means of layer-by-layer assembly (LbL). The properties of the obtained microcapsules were characterized by scanning electron microscopy, dynamic light scattering, infrared-, ultraviolet- and visible spectroscopy. According to the performed DLS measurements, an average hydrodynamic diameter ranged from 4 to 8 m and zeta-potential for all types of capsules was determined as -18 and -21 mV. BSA-FITC was encapsulated using this approach yielded a loading efficiency of 49 % protein. This value calculated for dexamethasone was of 38%. The microcapsules filled with an encapsulated drug may find applications in the field of biotechnology, biochemistry, and medicine.