High spatial efficiency single-particle trapping array based on a microfluidic device

2019 International Conference on Advanced Electronic Materials, Computers and Materials Engineering

无线电电子学;计算机科学;材料科学

Duan, Yu^1 ; Zhang, Weiguan^2 ; Peng, Zhengchun^1

College of Optoelectronic Engineering, Shenzhen University, Shenzhen

518061, China^1

College of Mechatronics and Control Engineering, Shenzhen University, Shenzhen

518061, China^2

关键词: High throughput;    Hydrodynamic forces;    Least flow resistance;    Micro-fluidic devices;    Side by sides;    Single particle;    Single-cell trapping;    Singlecell analysis;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/563/3/032044/pdf
DOI  :  10.1088/1757-899X/563/3/032044

来源: IOP

【 摘 要 】

High-throughput single cell trapping is critical for cell biology research. In this paper, a microfluidic device with micro-cup array is designed to achieve high-throughput single-particle trapping. Microspheres were aspired in the micro-cup array and immobilized in site by hydrodynamic force generated by the flow. Design of micro-cup is optimized based on "least flow resistance path" principle showing that higher volume flow ratio leads to better capturing efficiency. The result showed 100% capturing efficiency with flow ratio of 1.744. Also, by placing the micro-cup structure side-by-side in the channel, high-throughput of the microfluidic device is realized. 35 microspheres can be captured in the trapping area of 160×510 μm2 which is a promising tool for high-throughput single cell analysis.