Neural signal registration and analysis of axons grown in microchannels

3rd International School and Conference on Optoelectronics, Photonics, Engineering and Nanostructures (Saint Petersburg OPEN 2016)

Pigareva, Y.^1 ; Malishev, E.^2 ; Gladkov, A.^1,3 ; Kolpakov, V.^1 ; Bukatin, A.^2 ; Mukhina, I.^1,3 ; Kazantsev, V.^1 ; Pimashkin, A.^1

Department of Neuroengineering, Center of Translational Technologies, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia^1

Saint-Petersburg Academic University, Nanotechnology Research and Education Centre of the RAS, Saint-Petersburg, Russia^2

Central Research Laboratory, Cell Technology Department, Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia^3

关键词: Bioelectrical signals;    Electrical activities;    Extracellular recording;    Functional structure;    Fundamental mechanisms;    Micro-fluidic devices;    Neural network connectivities;    Neural signal processing;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/741/1/012057/pdf
DOI  :  10.1088/1742-6596/741/1/012057

来源: IOP

【 摘 要 】

Registration of neuronal bioelectrical signals remains one of the main physical tools to study fundamental mechanisms of signal processing in the brain. Neurons generate spiking patterns which propagate through complex map of neural network connectivity. Extracellular recording of isolated axons grown in microchannels provides amplification of the signal for detailed study of spike propagation. In this study we used neuronal hippocampal cultures grown in microfluidic devices combined with microelectrode arrays to investigate a changes of electrical activity during neural network development. We found that after 5 days in vitro after culture plating the spiking activity appears first in microchannels and on the next 2-3 days appears on the electrodes of overall neural network. We conclude that such approach provides a convenient method to study neural signal processing and functional structure development on a single cell and network level of the neuronal culture.