An assessment of the potential criticality hazard associated with isotope processing operations in the Hot Cell Facility has been accomplished in accordance with DOE-STD-3007-93. This assessment includes the consideration of the quantities, forms, and locations of fissile material that will exist in the HCF. Contingency analyses have been accomplished to evaluate the multiple events which could potentially result in a criticality event. These analyses included the effects of flooding and procedural errors. Validated calculational techniques have been used to evaluate all credible configurations of fissile material. A wide range of fissile arrays and hypothetical configurations were numerically analyzed to assess the potential for criticality. In all cases, the results indicated that fissile material arrays would remain subcritical in the HCF. The design features and administrative controls on which these analyses were based are identified.