Shotgun protein sequencing.

Faulon, Jean-Loup Michel ; Heffelfinger, Grant S.

Sandia National Laboratories

关键词: Digestion;    Modifications;    Dna;    99 General And Miscellaneous//Mathematics, Computing, And Information Science;    59 Basic Biological Sciences;   

DOI  :  10.2172/959081
RP-ID  :  SAND2009-3855
RP-ID  :  AC04-94AL85000
RP-ID  :  959081

美国|英语

来源: UNT Digital Library

【 摘 要 】

A novel experimental and computational technique based on multiple enzymatic digestion of a protein or protein mixture that reconstructs protein sequences from sequences of overlapping peptides is described in this SAND report. This approach, analogous to shotgun sequencing of DNA, is to be used to sequence alternative spliced proteins, to identify post-translational modifications, and to sequence genetically engineered proteins.