Functional characterization of dna repair proteins

Wilson, D M III

Lawrence Livermore National Laboratory

关键词: Dna Damages;    Dna;    Diseases;    Genetics;    Modifications;   

DOI  :  10.2172/15001995
RP-ID  :  UCRL-ID-137638
RP-ID  :  W-7405-ENG-48
RP-ID  :  15001995

美国|英语

来源: UNT Digital Library

【 摘 要 】

Genetic material (DNA) is susceptible to spontaneous decomposition, to attack by reactive chemicals produced naturally in cells, and to assault by environmental and food mutagens. DNA modifications can lead to permanent genetic changes that promote human disease. To combat the deleterious effects of DNA damage, organisms are equipped with DNA repair systems. The focus of our investigations has been to elucidate the details of mammalian DNA repair. These studies have provided important insights into the relationship of DNA repair to human disease and the genetic factors that contribute to individual susceptibility to the harmful effects of environmental mutagens (e.g. ionizing radiation), and have established a framework for designing more effective anti-cancer treatment schemes. To investigate questions related to DNA repair, its mechanism(s) and linkage to disease development, the authors are employing an array of molecular, cellular, biochemical and structural approaches. These complementary experimental techniques have permitted a detailed analysis into various processes of human DNA repair.