FY05 LDRD Fianl Report Investigation of AAA+ protein machines that participate in DNA replication, recombination, and in response to DNA damage LDRD Project Tracking Code: 04-LW-049

Sawicka, D ; de Carvalho-Kavanagh, M S ; Barsky, D ; Venclovas, C

Lawrence Livermore National Laboratory

关键词: Dna Damages;    Dna;    Genetics;    Recombination;    59 Basic Biological Sciences;   

DOI  :  10.2172/897962
RP-ID  :  UCRL-TR-226563
RP-ID  :  W-7405-ENG-48
RP-ID  :  897962

美国|英语

来源: UNT Digital Library

【 摘 要 】

The AAA+ proteins are remarkable macromolecules that are able to self-assemble into nanoscale machines. These protein machines play critical roles in many cellular processes, including the processes that manage a cell's genetic material, but the mechanism at the molecular level has remained elusive. We applied computational molecular modeling, combined with advanced sequence analysis and available biochemical and genetic data, to structurally characterize eukaryotic AAA+ proteins and the protein machines they form. With these models we have examined intermolecular interactions in three-dimensions (3D), including both interactions between the components of the AAA+ complexes and the interactions of these protein machines with their partners. These computational studies have provided new insights into the molecular structure and the mechanism of action for AAA+ protein machines, thereby facilitating a deeper understanding of processes involved in DNA metabolism.